胃炎颗粒质量标准研究  

Study on Quality Standard of Weiyan Granules

作　　者：胡斌[1] 林彩[1] 赵映兰[1] 邓莉[1] 

机构地区：[1]第三军医大学西南医院药学部,重庆400038

出　　处：《中国药业》2009年第23期28-29,共2页China Pharmaceuticals

摘　　要：目的建立胃炎颗粒中芍药苷的含量测定方法。方法采用反相高效液相色谱(RP-H PLC)法,色谱柱为C18柱(250mm×4.6mm,5μm),流动相为乙腈-水(19∶81),检测波长229nm,流速1.0mL/m in,柱温30℃,进样量10μL。结果芍药苷质量浓度在2.45～12.24μg/m L范围内与峰面积线性关系良好,平均回收率为99.80%,RSD=0.99%(n=9)。结论RP-HPLC法操作简单,检测结果准确可靠,可用于胃炎颗粒的质量控制。Objective To develop a method for determining the content of paeoniflorin in Weiyan Granules. Methods RP-HPLC was used. The chromatographic system consisted of C18 column （250 mm ×4.6 mm, 5 μm） and the mobile phase of MeCN-H20 （19：81） with the detection wavelength at 229 nm, the flow rate was 1.0 mL/min. The column temperature was 30 ℃ the sample size was 10 μL. Results The linear range for paeoniflorin was 2. 45 - 12. 24 μg/mL（ r=0. 9999）, and the average recovery rate was 99.80%, RSD = 0. 99% （ n = 9）. Conclusion Without complicated sampling procedure, the method is simple, rapid and accurate, which can be used to control the quality of this pharmaceutical formulation.

关 键 词：芍药苷 胃炎颗粒 反相高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]