苜蓿斑蚜为害对5种苜蓿品种(系)PAL、POD、PPO酶活性的影响  

The effects of Therioaphis trifolii on the activities of PAL,POD and PPO in five alfalfa varieties

作　　者：程璐[1] 贺春贵[1] 胡桂馨[1] 王森山[1] 朱亚灵 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室／中-美草地畜牧业可持续发展研究中心,兰州730070 [2]甘肃省兰州市园林科学研究所,730070

出　　处：《植物保护》2009年第6期87-90,共4页Plant Protection

基　　金：国家科技支撑计划项目(2006BAD04A04-01);甘肃省教育厅科研项目(0902-10)

摘　　要：在室内将苜蓿斑蚜人工接种在不同抗性级别的苜蓿品种(系)幼苗上,苜蓿斑蚜为害后的3、71、0 d分别测定了5个品种(系)叶片中苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)3种酶活性。结果表明,在试验期内,所有品种(系)叶片中的PAL活性在接虫后随着时间的增加而升高;PPO活性随天数增加呈下降趋势;POD与PAL酶的情况相同,随着为害时间的延长不断升高。HA-3感蚜后3种酶活性明显高于其他品种。Therioaphis trifolii was manually inoculated to five varieties （strains） alfalfa seedlings with different resistance levels indoors, and the enzymatic activities of phenyialanine ammonia-lyase （PAL）, poiyphenol oxidase （PPO） and peroxidase （POD） were determined. The results showed that, during the trial period, the activities of PAL and POD in leaves of all varieties increased remarkably with increasing days after inoculation, while the activity of PPO decreased with the treatment days increasing. After inoculation, the activities of the three enzymes in HA-3 were obviously higher than that of other varieties.

关 键 词：苜蓿 抗性 苯丙氨酸解氨酶 多酚氧化酶 过氧化物酶 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]