加拿大进境大豆上检出菜豆荚斑驳病毒  

Detection of Bean pod mottle virus in soybean imported from Canada

作　　者：沈建国[1] 王念武[1] 翁瑞泉[1] 黄可辉[1] 郭琼霞[1] 

机构地区：[1]福建出入境检验检疫局,福建省检验检疫技术研究重点实验室,福州350001

出　　处：《植物保护》2009年第6期127-129,共3页Plant Protection

基　　金：福建省自然科学基金项目(B0610001,2006J0047);福建出入境检验检疫局科技项目(FK2007-25);福建科技重大专项专题项目(2006NZ0002-1)

摘　　要：本研究采用DAS-ELISA、IC-RT-PCR及序列测定方法,对加拿大进境大豆种子进行菜豆荚斑驳病毒(Beanpod mottle virus,BPMV)和大豆花叶病毒(Soybean mosaic virus,SMV)检测,结果表明,BPMV的DAS-ELISA和IC-RT-PCR检测结果均为阳性,且PCR扩增产物序列与已报道的BPMV基因序列相似性达97%以上,而SMV的DAS-ELISA和IC-RT-PCR检测结果都为阴性。综合血清学、分子生物学检测结果,确认该批大豆携带有菜豆荚斑驳病毒。The presence of BPMV and SMV in soybean seeds imported from Canada was determined using DAS- ELISA, IC-RT-PCR and sequence analysis. The results showed that both DAS-ELISA and IC-RT-PCR were positive for BPMV, and the nueleotide sequence of BPMV PCR product had more than 97% similarity to previously reported genomic sequence. However, negative DAS-ELISA and IC-RT-PCR results were obtained for SMV. According to the results of serological and molecular biological methods, it was confirmed that BPMV was present in tested soybean imported from Canada.

关 键 词：菜豆英斑驳病毒 检测 DAS-EUSA IC-RT-PCR 

分 类 号：S41-31[农业科学—植物保护]