利用性控精液体外生产奶牛早期胚胎  

In vitro Production of Early Dairy Cattle Embryos by Using X-bearing Sperm

作　　者：秦国嵩[1] 李贺娟[1] 梁鸿斌[1] 郭勇[1] 倪和民[1] 刘云海[1] 

机构地区：[1]北京农学院动物科技系,北京102206

出　　处：《中国畜牧杂志》2009年第23期20-23,共4页Chinese Journal of Animal Science

基　　金：北京市教育委员会科技发展计划重点项目(KZ200510020013);北京市教育委员会科技发展计划面上项目(KM200710020006);2007年度教育部留学人员科技活动择优资助项目(启动类)

摘　　要：实验进行了精子处理方法、洗精液中添加抗生素种类及浓度、常规培养小滴(50μL)中入胚数的筛选,按照不同荷斯坦公牛个体分选的性控精液对体外生产的牛早期胚胎发育潜力的影响。结果表明:离心法处理性控精液效果最优。利用经过分选的性控精液进行体外受精(IVF)后都较易导致污染,且受精后的体外胚胎发育效果都较差。在洗精液中添加10μL/mL头孢哌酮钠的IVF胚胎体外发育较好,囊胚率达到16.9%;且在常规培养小滴中适当减少入胚数,能有效抑制利用性控精液制备的奶牛IVF胚胎自身对培养微滴的污染。利用直接离心法处理精液,在洗精液中添加头孢哌酮钠10μL/mL,可以成功进行牛性控精子的体外受精,但目前的受精效果低于用普通精子的体外受精效果。This study was carried out to compare, the different processing methods for X-bearing sperm screening, antibiotic type and density screening in sperm-talp screening the optimal number of embryos in general culture of drop (50 μL) and compare the effect of early embryonic development potential by using different individual Holstein bull′s X-bearing sperm. Results indicated that the X-bearing sperm treated by centrifugation can attain optimal effect. It was possible to cause pollution in vitro fertilization by using different bull′ s X-bearing sperm, and their embryonic development in vitro were also at a very low level. In vitro embryonic development by using X-bearing sperm in medium with 10 μL/mL Cefoperazone Sodium, was much better than others antibiotic (the blastocyst rate 16.9%); and reducing the number of embryo on the general culture drop could inhibit the pollution which caused by X-bearing sperm IVF embryo. The in vitro fertilization could be adaptable while the bovine X-bearing sperms were treated by direct centrifuge processing and adding with 10 μL/mL Cefoperazone Sodium, but the effect of such kind of fertilization in vitro was much lower than that of normal one.

关 键 词：胚胎 体外受精 性控精液 奶牛 

分 类 号：S823.3[农业科学—畜牧学]