蟹源益生芽孢杆菌的筛选  

Screening of Chinese mitten crab(Eriocheir sinensis)source probiotic bacillus strains

作　　者：郝向举[1] 李义[1] 孙汉[1] 张红英[1] 王玥[1] 

机构地区：[1]苏州大学基础医学与生物科学学院

出　　处：《中国饲料》2009年第22期11-14,共4页China Feed

基　　金：苏州市科技攻关项目(SNG-0714)

摘　　要：本试验根据菌体对高温和低pH的耐受性、产酶能力及对病原菌的抑菌效果,对中华绒螯蟹肠道中的芽孢杆菌进行了筛选。结果表明,在筛选到的3株芽孢杆菌中,ESB3菌株抑制中华绒螯蟹病原菌嗜水气单胞菌CL99920菌株的能力最强,产蛋白酶和淀粉酶的能力也较好,在肉汤培养基初始pH3.0的条件下培养2h时,其存活率为(32.56±2.87)%,90℃水浴处理10min存活率为(68.54±8.28)%;浓度低于1×108CFU/mL对中华绒螯蟹无毒害作用,1×107CFU/mL的ESB3能提高中华绒螯蟹对嗜水气单胞菌感染的抵抗力;经形态学和生理生化反应鉴定,ESB3为地衣芽孢杆菌。Bacillus from intestine of Chinese mitten crab （Eriocheir sinensis ）were screened according to their tolerance of high temperature ,low pH ,enzyme producing features and activity of inhibition against pathogens in the experiment.The results indicated that Bacillus ESB3 had the best effect of inhibition against Aeromonas hydrophila CL99920 and better capability of producing protease and amylase.On the condition of initial pH 3.0 in Nutrient Broth medium and water-bath of 90 ℃ for 10 min,its survival rate were （32.56±2.87）% and （68.54±8.28）%,respectively.h was safe for the crab when they were challenged by the immersion concentration of ESB3 below 1×10^8 CFU/mL,and it could enhance the resistance of crab against A.hydrophila CL99920 when the immersion concentration of ESB3 was 1×10^7 CFU/mL.By morphological physiological and biochemical tests,it confirmed that ESB3 was Bacillus licheniformis.

关 键 词：中华绒螯蟹 益生菌 芽孢杆菌 筛选 鉴定 

分 类 号：S811.6[农业科学—畜牧学]