利用GiemsaC-带和45SrDNAFISH的方法鉴定百合杂种  

Identification of Lilium hybrid by Giemsa C-banding and 45S rDNA FISH

作　　者：胡凤荣[1] 刘光欣[1] 罗凤霞[2] 席梦利[1] 徐进[1] 施季森[1] 

机构地区：[1]南京林业大学,林木遗传和生物技术省部共建教育部重点实验室,江苏南京210037 [2]金陵科技学院,江苏南京211169

出　　处：《南京林业大学学报（自然科学版）》2009年第6期30-34,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业局“948”创新项目(2007-4-06);上海农业科技攻关项目(2006-4-1)

摘　　要：利用Giemsa C-带和染色体荧光原位杂交(45S rDNA FISH)的方法对‘Royal Lace’בHigh Class’的杂种后代分别进行了鉴定。结果表明:‘Royal Lace’的染色体数2n=3x=36,‘High Class’的染色体数2n=2x=24。杂种后代的染色体数出现2n=3x=36和2n=4x=48两种类型。经Giemsa C-带和45S rDNA FISH方法对两个亲本和具2n=4x=48的杂种后代分析结果表明,杂种后代的根尖染色体C-带可观察到来自双亲的可以特异追踪的染色体带纹。FISH分析表明,杂种后代中分别有两条来自‘Royal Lace’和‘High Class’的染色体。两个亲本的荧光原位杂交点数分别为10和9。杂种后代染色体为2n=36的个体有14个杂交信号,可以确定两条来自‘Royal Lace’,另外3条来自‘High Class’。杂种后代中2n=48的个体的杂交信号点为19,从而证实所获得的杂种后代的真实性。同时,对于2n=48,而杂交信号为19的多倍体起源于未减数分裂的2n雄配子体的可能性进行了探讨。综合研究结果表明,Giemsa C-带和45S rDNA FISH方法可以准确地对百合的杂种真实性进行鉴定。In this study, hybrid plants from the crosses hetween ＇ Royal Lace＇ x ＇ High Class＇ were analyzed with Gi- emsa C - banding and 45S rDNA FISH. The results showed that, the number of chromosomes was 2n = 3x = 36 for ＇Royal Lace＇ , 2n=2x=24 for ＇High Class＇ and2n=3x=36 or2n=4x--48 for two different hybrids, respectively. The special C -banging patterns of two parents can be found in their hybrids. By FISH analysis, 14 hybridization locies were detected in the hybrids of 2n = 36. Two chromosomes from ＇ Royal I.ace＇ and three chromosomes from ＇ High Class＇ can be identified clearly. And there were 19 hybridization locies to be distinguished for 2n = 48 hybrids,which also suggested that these hybrids might relate to be the contributed by unreduced male gametes （2n）. All of these evidences clearly confirmed that, the plants were true hybrids.

关 键 词：百合杂种 染色体 C-带分析 染色体荧光原位杂交 

分 类 号：Q948[生物学—植物学]