蝉拟青霉分生孢子萌发的影响因子  

Factors Affected Conidia Germination of Paecilomyces cicadae

作　　者：李忠[1,2] 邹晓[3] 刘爱英[3] 金道超[4] 

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州大学,贵州山地农业病虫害重点实验室,贵州贵阳550025 [3]贵州大学真菌资源研究所,贵州贵阳550025 [4]贵州大学贵州山地农业病虫害重点实验室,贵州贵阳550025

出　　处：《贵州农业科学》2009年第11期86-89,共4页Guizhou Agricultural Sciences

基　　金：贵州大学人才基金项目"中药材白术病虫害生物防治技术研究"[贵大人基合字(2008)031];国家自然科学基金项目"虫生真菌蝉拟青霉毒力稳定性研究"(30360064)

摘　　要：研究了不同营养、温度、湿度、光照、pH值等条件对蝉拟青霉LB菌株分生孢子萌发的影响。结果表明：2％葡萄糖＋1％蛋白胨溶液制成孢子悬浮液的萌发率最高达95.09％；25-27℃是蝉拟青霉LB菌株分生孢子萌发的适宜温度条件；分生孢子萌发所需湿度为90％-100％，当湿度低于90％时萌发受到抑制；分生孢子在pH6-7的中性至弱酸环境下萌发最好；光照对孢子萌发无影响；紫外线对孢子有明显的杀伤作用，随紫外线照射时间的延长，孢子萌发率明显降低，经紫外线照射后孢子有萌发迟缓的现象。Effects of different nutrition, temperature, moisture, light, and pH were studied on conidia germination of Paecilomyces cicadae LB. The results showed that the nutrition （2% glucose ＋ 1% peptone）strongly promoted conidia germination, and reached the highest germination rate 95.09%. The optimum temperature for the germination of the spore was 25-27℃. The relative humidity for germination was between 90%-100%, while less than 90% spore could not germinate. The suitable pH was 6-7 for spore germination. There was no effect from light for spore germination. UV obviously had killing effects on spore, the germination rate of spore decreased evidently along with the longer exposure time, spore had retarded germination when treated by UV, with germination rate 78.10% in 24 hours treated 20 min, 52.73% for 40 min, and 23.36% for 60 min.

关 键 词：蝉拟青霉 分生孢子 萌发率 

分 类 号：S482.39[农业科学—农药学]