奶牛场蚊蝇体内细菌种类的调查  

Investigation of Bacteria Species in Mosquitoes and Flies in Dairy Farms

作　　者：李德光[1] 王开功[1,2] 周碧君[1,2] 文明[1,2] 方英 刘芳[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025 [3]贵阳市动物疫病预防控制中心,贵州贵阳550001

出　　处：《贵州农业科学》2009年第11期111-112,共2页Guizhou Agricultural Sciences

基　　金：国家科技部支撑计划子课题"疫病防控技术集成与推广"(2007BAD53B03);贵州省科技厅重大专项子课题"种猪场主要疫病免疫程序与生物安全程序研究"[黔科字重大专项(20086011)];贵州省农业厅兽医科技计划项目"2009年贵州省主要动物疫病免疫监测研究"(200909);贵州省教育厅自然科学研究项目"贵阳市规模奶牛场隐性乳房炎病原学研究及公共卫生意义"(黔教科2006303);贵阳市农业局项目"贵阳市养殖小区主要奶牛疾病的检测和综合防治"(Z074003)

摘　　要：为了解奶牛场环境中蚊蝇体内携带细菌的情况,从贵阳市4个规模化奶牛场采集蚊子和苍蝇各34只进行细菌的分离与鉴定。结果表明:从21只蚊子和33只苍蝇体内分离到细菌,检出率分别为61.76%和97.1%;在分离的53株细菌中,鉴定为大肠埃希氏菌的有5株、克雷伯氏菌3株、志贺氏菌4株、肠杆菌1株、表皮葡萄球菌6株、腐生葡萄球菌2株和未定种32株。结果可为奶牛场细菌性疾病的综合防治提供科学数据。To study the bacteria carried by mosquitoes and flies in diary cow farm, 34 mosquitoes and flies were collected respectively from 4 large-scale dairy farms in Guiyang city. The results showed that bacteria were isolated from 21 mosquitoes and 33 flies, with detection rates 61.76% and 97.1%. 5 strains of E. coli, 3 strains of Lebsiella, 4 strains of Shigella, 1 strain of Enterobacteriaceae, 6 strains of Staphylococcus epiderrnidis, 2 strains of Staphylococcus saprophyticus and 32 undetermined species were identified in the 53 strains of bacteria. The study could provide a scientific reference for bacteriosis integrated control in dairy farms.

关 键 词：奶牛场 蚊蝇 细菌 分离鉴定 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]