RGD结合型阿克拉霉素A脂质体的抑瘤作用  

Inhibiting Effect of Aclacinomycin A Liposomes Conjugated with RGD Peptide

作　　者：李占山[1] 周瑞华[2] 李少燕[1] 梁秀敏[1] 佟建敏[1] 

机构地区：[1]华北煤炭医学院附属医院药剂科,河北唐山063000 [2]华北煤炭医学院预防医学系

出　　处：《中国药师》2009年第12期1702-1704,共3页China Pharmacist

基　　金：河北省医学研究重点课题(No:04037)

摘　　要：目的:观察RGD多肽结合型阿克拉霉素A长循环脂质体(RGD-ACM-A-liposome)对动物实验肿瘤的抑制作用。方法:以逆相蒸发法制备ACM-A长循环脂质体,并通过反应与RGD基元多肽进行共价键结合.然后静脉注射于荷瘤小鼠体内,之后处死动物,解剖肿瘤,计算并比较抑瘤率及生命延长率。结果:RGD多肽结合型阿克拉霉素A长循环脂质体对小鼠S180和EAC腹水癌具有较好的抑制作用。结论:RGD多肽结合型阿克拉霉素A长循环脂质体可能通过导致血管凋亡以及局部释放抗癌药杀灭癌细胞,对新生肿瘤具有抑制与治疗作用。Objective ： To observe the inhibiting effect of Aclacinomycin A PEG-liposomes conjugated with RGD peptide （ RGD- ACM-A-liposome） on tumor cells in experimental animals. Method： RGD-ACM-A-Liposome was prepared by reverse phase evaporation method and reacted with GRGDS peptide. RGD-ACM-A-liposomes were intravenously injected into tumor bearing mice. After that, mice were sacrificed and tumors were dissected and checked. The tumor inhibiting rate and prolongation in life span were calculated. Result： RGD-ACM-A-Liposome has good tumor inhibiting effect on S180 and EAC tumor bearing mice, Conclusion： RGD-ACM-A-Li- posome can be inhibit on growing tumor by causing apoptosis of its new growing blood vessel endothelial cells and killing cancer cells.

关 键 词：RGD 多肽结合型阿克拉霉素A长循环脂质体 制备 肿瘤凋亡 

分 类 号：R965.1[医药卫生—药理学]