柱前衍生-高效液相色谱法测定人血清中丙戊酸钠的浓度  

Determination of Sodium Valproate in Serum by HPLC with Precolumn Derivatization

作　　者：冯惠平[1] 王璟[1] 陈宜峰[1] 李碧峰[1] 

机构地区：[1]福建医科大学附属漳州市医院药剂科,福建漳州363000

出　　处：《中国药师》2009年第12期1755-1757,共3页China Pharmacist

摘　　要：目的:建立快速柱前衍生-高效液相色谱法测定人血清中丙戊酸钠(VPA)的浓度。方法:血样经酸化后用正戊烷提取,以环已烷羧酸为内标,w-溴苯乙酮为衍生化试剂,采用Agilent HC-C_(18)柱(250mm×4.6mm,5μm),流动相为甲醇-水(73:27),检测波长为262nm,流速1.0ml·min^(-1),柱温40℃。结果:血清中VPA浓度在10.12～182.16μg·ml^(-1)范围内具有良好的线性关系(r=0.9997),平均相对回收率为99.17%,日内、日间RSD均小于6%,最低定量浓度为10.12μg·ml^(-1)。结论:本法简便、快速、准确,适合临床常规血药浓度监测。Objective ： To establish a rapid analysis method for the determination of sodium valproate in serum by HPLC precolumn derivatization. Method： The serum sample was extracted after acidification. Cyelohexanecarboxylic acid was selected as an internal standard, w-bromoaeetophenone was selected as derivative reagent, the analytical column was agilent HC-C18 column （250 mm×4.6 mm,5μm） , The mobile phase was methanol-deionized water （73：27）, The detection wavelength was 262nm , the flow rate was 1.0ml.min^-1 and the temperature of column was set at 40℃. Result： The calibration curve was linear in the range of 10.12 - 182.16 μg. ml^-1 （r = 0. 999 7 ） , the average recovery was 99.17% , RSD values of within-day and between-day were less than 6% , The lowest limit of determination of sodium valproate was 10.12μg. ml^-1. Conclusion ： The method is convenient, rapid, accurate and suitable for therapeutic VPA monitoring.

关 键 词：柱前衍生 高效液相色谱法 丙戊酸钠 

分 类 号：R965.1[医药卫生—药理学]