单核细胞TLR7／8表达与HIV-1感染疾病进程关系的研究  

Study on the relationship between the expression of TLR7/8 in monocytes and the disease progression of HIV-1 infection

作　　者：年华[1] 耿文清[1] 崔华露[1] 张子宁[1] 张旻[1] 韩晓旭[1] 赵彬[1] 胡清海[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中华微生物学和免疫学杂志》2009年第11期992-996,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家科技重大专项课题资助项目（2008ZX10001-001）;国家重点基础研究发展计划（973计划）（2006CB504206）;辽宁省医学重点专科项目（辽卫函字[2007]499号）

摘　　要：目的对HIV—1感染者单核细胞TLR7／8表达水平及与疾病进展的相关性进行研究，探讨单核细胞TLR7／8在HIV-1疾病进程中的作用。方法选取63名HIV-1感染者及18名健康对照，应用MACS磁珠分选法纯化CD14^＋单核细胞，用2．5μg/ml R848刺激单核细胞TLR7／8，QIAGEN公司的RNA提取试剂盒提取单核细胞总RNA，实时定量RT-PCR测定TLR7／8的mRNA表达。结果HIV-1感染者单核细胞TLR7和TLR8的表达水平与CD4^＋T细胞显著正相关（r=0．614，P〈0．01；r=0．419．P〈0．01）。TLR7 mRNA表达：缓慢进展组明显高于HIV感染组、AIDS组和健康对照组（P〈0．05），HIV感染组明显高于AIDS组（P〈0.05）；TLR8 mRNA表达：缓慢进展组明显高于AIDS组（P〈0．05）。体外用R848刺激TLR7后表达显著下调，而TLR8的表达稳定。结论AIDS疾病进程中HIV．1感染者单核细胞TLR7／8表达水平明显下降，TLR7的表达水平下降更显著。Objective To examine the expression of TLR7/8 in monocytes purified from HIV-1 infected individuals and to study its association with disease progression. Methods Sixty-three HIV-1 infected individuals and 18 normal controls were enrolled. Monocytes were purified by MACS system and RNA of them was extracted by RNA mini kit of QIAGEN company. TLR7/8 expression was tested by real-time RT-PCR with ABI7500. Results It was found that the expression of TLR7 was strongly correlated with absolute CD4 count （r = 0.614, P 〈 0.01 ） , so was TLR8 （r = 0.419, P 〈 0.01 ）. The expression of TLR7 in slow progressor （SP） group was higher than that in HIV-1 infected patients group, AIDS patients group and normal group （ P 〈 0.05 ）. HIV group and normal group were strongly higher than AIDS group （ P 〈 0.05 ）. It was no singnificant differentiation of expression of TLR7 between HIV infection group and normal control group. The expression of TLR8 in SP group and normal group were significantly higher than that in AIDS group （P 〈 0.05 ）. The expression of TLR8 was no singnificantly difference between SP group and HIV group or normal control group, so was it between HIV group or normal control group and AIDS group. Conclusion The expression of TLR7/8 in monocytes from HIV-1 infected patients significantly correlated with disease progression.

关 键 词：HIV-1 单核细胞 TLR7 TLR8 

分 类 号：R512.91[医药卫生—内科学]