胸腺五肽乙酯的抗应激作用研究  

Study on Anti-stress Activity of Thymopentin Ethyl Ester

作　　者：迟强[1] 许鹏[1] 杜丽娜[2] 卢山[1] 栾中山[1] 徐珺洋[1] 王黎黎[1] 杨志[1] 

机构地区：[1]解放军第406医院215临床部,大连116041 [2]解放军军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《医学研究杂志》2009年第11期70-73,共4页Journal of Medical Research

摘　　要：目的考察胸腺五肽乙酯的抗应激作用。方法 (1)抗热应激作用:昆明系小鼠,共6组,在22℃条件下适应2天。除空白组外,其他小鼠放于42℃恒温孵箱中作用1h。在此期间禁水禁食。空白组和模型组皮下注射生理盐水0.2ml,试验组给予胸腺五肽乙酯生理盐水溶液,高剂量组2mg/kg、中剂量组0.2mg/kg、低剂量组0.02mg/kg,阳性对照组给予TP5 0.2mg/kg。(2)抗慢性应激作用:昆明系小鼠,共6组,置于22℃环境中,自由食水,可以自由活动。除空白组外,其余小鼠接受21天不同的应激刺激,每日1次,平均每种刺激3次。于每日进行刺激前1h皮下注射给药,空白组和模型组每天给予生理盐水0.2ml,试验组给予胸腺五肽乙酯生理盐水溶液,高剂量组2mg/kg、中剂量组0.2mg/kg、低剂量组0.02mg/kg,阳性对照组给予TP5 0.2mg/kg。分别在热应激模型和慢性应激小鼠模型上,通过放射免疫分析法测定血浆皮质醇、IL-2含量,以羟胺法测定血浆SOD水平。结果胸腺五肽乙酯能抑制应激小鼠的皮质醇增高效应,并提高血浆IL-2水平和SOD活力,与模型组和阳性对照组相比有显著性差异(P<0.05)。结论胸腺五肽乙酯具有明显的抗应激作用。Objective To investigate the anti - stress activity of thymopentin ethyl ester. Methods Anti - stress activity was evalu- ated by heat stress model and chronic uncontrolled stress model. Heat stress model：of 60 KM mice were divided into six groups. Except the controlled group, the other mice were maintained in 42℃ for 1 h. Chronic uncontrolled stress model：of 60 KM mice were divided into six groups. Except the controlled group, the other mice were given three different stimulations once a day for continuous 21d. The con- trolled group and model group were injected saline 0.2ml, and the three test groups were respectively injected thympentin ethyl ester at 2mg/kg,0. 2mg/kg, 0.02mg/kg subcutaneously. The positive controlled mice were given thymopentin 0.2mg/kg subcutaneously. At the end of the experiment, plasma corticosteroid, IL - 2 and SOD were determined according to the kit instructions. Results The activities of thymopentin ethyl ester in suppressed corticosteroid up - regulation and the elevated plasma IL - 2 and SOD level were more significant than those of thymopentin（ P 〈 0.05）. Conclusion Thymopentin ethyl ester showed obvious anti - stress activity.

关 键 词：胸腺五肽乙酯 免疫增强 应激 

分 类 号：R285.5[医药卫生—中药学] R512.62[医药卫生—中医学]