肠易激综合征慢急性联合应激动物模型的建立及其感觉、动力和心理行为的评价  

The establishment of an animal model of gut-brain interaction in irritable bowel syndrome for the evaluation of visceral sensation, motility and psychological behavior

作　　者：吕红[1] 钱家鸣[1] 金光亮[2] 周东丰[3] 沈渔邦[3] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院消化科,100730 [2]北京中医药大学 [3]北京大学第六医院

出　　处：《中华内科杂志》2009年第12期1035-1039,共5页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金资助项目（30470795）

摘　　要：目的建立行为、感觉和动力等多因素作用的肠易激综合征（IBS）脑肠交互作用的动物模型。方法在慢性轻度不可预见性应激（GUMS）基础上与束缚应激（WRS）刺激相结合，建立多因素相互作用的慢急性联合应激动物模型（CACS），检测一定时间内大鼠动力（排便颗粒、排便时间）、感觉（自发性腹外斜肌收缩）和行为／心理（糖水摄取量、体重）等指标改变。同时采用Western blot方法检测大鼠海马和前额叶皮质G蛋白亚型变化。结果（1）与应激前比较，CACS组大鼠排便颗粒数增多，直肠内玻璃小球排出时间缩短，不同气囊容量下腹部收缩次数增多、糖水摄取量减少；而CUMS组排便行为无明显改变；WRS组糖水摄取量无明显改变。（2）与对照组比较，CACS组和CUMS组大鼠脑部G蛋白某些亚型表达减少（P〈0．05），而WRS组各G蛋白亚型无明显变化。结论CACS是一种新型的脑肠交互作用模型，可以较好地模拟人类IBS部分症状。Objective To develop a gut-brain interaction animal model of IBS which combines multiple factors including behavior, visceral sensation and motility. Methods Setting up a muhifactor interactional animal model （chronic acute combining stress model, CACS） based on a chronic unpredictable mild stress model of depression （CUMS） while combined with wrap restraint stress （ WRS ）, changes of some indexes were recorded including motility （granules of defecating, time of defecating）, visceral sensitivity （spontaneous contraction of abdominal striated muscles ） and behavior/mind （sucrose consumption, body weight）. G protein subunits were measured by Western blot in both hippocampus and prefrontal cortex simultaneously. Results （ 1 ） Compared with the state before stress given, defecating granules increased, defecating time of glassie from rectum shorten, number of abdominal contraction increased, and sucrose consumption decreased in CACS, however, neither significant change was found on defecating behavior in CUMS nor on sucrose consumption in WRS; （ 2 ） Compared with the control group, some G protein submits expression decreased in both CACS and CUMS （P 〈 0. 05）, while no significant changes of any G protein subunits were found in WRS. Conclusion The CACS animal model was a new, brain-gut interaction model, which can mimic part of human symptoms of IBS very well.

关 键 词：肠易激综合征 模型 动物 应激 GTP结合蛋白质类 

分 类 号：R686[医药卫生—骨科学]