负载VEGF聚合物修饰铂金微弹簧圈的实验研究  

Preparation and property of platinum microcoil modified by a copolymer-VEGF conjugate

作　　者：汪求精[1] 孙新林[1] 姬斌[1] 祝爱萍[2] 全大萍[2] 

机构地区：[1]南方医科大学珠江医院神经外科,广东广州510282 [2]中山大学化学与化学工程学院,广东广州510275

出　　处：《南方医科大学学报》2009年第11期2171-2174,共4页Journal of Southern Medical University

基　　金：国家"十一五"科技支撑项目分课题(2006BAI01A12);广东省科技计划项目(2007B031003011);广州市科技计划项目(2006J1-C0081)

摘　　要：目的构建一种负载血管内皮生长因子(VEGF)的聚合物修饰铂金微弹簧圈表面,考察其表面特征及VEGF缓释性能。方法将4COOH-P(DLLA-co-TMC)聚合物溶液涂覆铂金微弹簧圈,固定肝素,利用肝素与VEGF的特异作用实现负载和控制释放VEGF。扫描电镜观察铂金微弹簧圈的表面结构,利用酶联免疫吸附方法(ELISA)测定微弹簧圈表面的VEGF缓释性能。结果铂金弹簧圈表面成功涂覆4COOH-P(DLLA-co-TMC)聚合物,通过聚合物的肝素化,使VEGF负载于铂金微弹簧圈表面,VEGF的释放量在测试周期内呈现明显的积累递增趋势,整个过程无明显的突释期。结论利用共聚物4COOH-P(DLLA-co-TMC)可实现在铂金微弹簧圈表面负载和控制释放VEGF的目的,赋予铂金微弹簧圈一定的生物活性。Objective To prepare a platinum microcoil coated with polymers and vascular endothelial growth factor（VEGF）,and evaluate its surface characteristics and property of sustained VEGF release.Methods The surface of the platinum microcoils（GDC） were modified by coating P（DLLA-co-TMC） copolymer and immobilizing heparin on the surface of GDC.VEGF was then loaded onto the surface of GDC and the controlled release of VEGF within GDC was achieved.The morphology was observed by scanning electron microscope,and the sustained release of VEGF was evaluated by enzyme-linked immunosorbent assay（ELISA）.Results Platinum coils were prepared by successive deposition of P（DLLA-co-TMC） copolymer and anionic heparin,and VEGF was immobilized through affinity interaction with heparin.The accumulative release of VEGF increased obviously during the entire testing period without burst release.Conclusion The use of P（DLLA-co-TMC） copolymer allows immobilization of VEGF on the platinum coils for controlled VEGF release,and improves the biological property of the coils.

关 键 词：铂金弹簧圈 4COOH-P(DLLA-co-TMC) 血管内皮生长因子 控制释放 

分 类 号：R318.0[医药卫生—生物医学工程]