大鼠宫颈MMP-2、MMP-8动态表达的研究  

Dynamic expression of matrix metalloproteinases 2 and 8 in rat cervix

作　　者：尹春艳[1] 毛邱娴[2] 罗姣红[2] 

机构地区：[1]广东省第二人民医院产科,广东广州510317 [2]广东医学院,广东湛江514023

出　　处：《南方医科大学学报》2009年第11期2205-2207,2210,共4页Journal of Southern Medical University

基　　金：广东省医学科学研究基金(A2002547)

摘　　要：目的通过检测大鼠宫颈基质金属蛋白2(MMP-2)、基质金属蛋白8(MMP-8)的动态表达,探讨胶原降解与宫颈成熟的关系。方法清洁级大鼠分为未孕动情间期、妊娠10d、妊娠16d、妊娠19d和分娩后即刻组,每组各6只。宫颈称湿重,HE常规染色显示宫颈大体结构,VG染色进行胶原纤维和肌纤维显色,免疫组织化学染色显示MMP-2、MMP-8。结果(1)HE染色显示大鼠宫颈主要由大量纤维(胶原纤维和肌纤维)组成,分娩后即刻组宫颈间质见少量中性粒细胞,其余组未见中性粒细胞。(2)临产前宫颈湿重已有增加,至分娩后即刻增加更明显。(3)宫颈纤维组织由胶原纤维和平滑肌纤维组成,所占比例无明显差别。分娩后即刻组胶原纤维和肌纤维排列较非分娩时松散。(4)MMP-2(棕染)在所有组别间质均有表达,鳞状上皮无表达,胶原纤维、平滑肌纤维、上皮及少量血管壁呈阳性表达;妊娠19d和分娩后即刻组阳性表达的密度较其他组增强。(5)分娩后即刻组MMP-8染色见间质散在中性粒细胞棕染,其他组的组织或细胞均未见阳性表达。结论宫颈成熟后,宫颈纤维组织松散、断裂,中性粒细胞由外源性血管浸润到宫颈间质,这一变化在分娩时尤其明显;MMP-2、MMP-8这两种不同来源的基质金属蛋白酶共同降解了宫颈纤维,导致宫颈软化、扩张。Objective To elucidate the relationship between collagen degradation and cervical ripening by detecting dynamic expressions of matrix metalloproteinases 2（MMP-2） and 8（MMP-8） in rat cervix.Method SPF rats were divided into 5 groups（n=6）,namely non-pregnancy estrus interval group,gestational days 10,16,and 19 groups,and immediately postpartum group.The wet weight of the cervix was measured and HE staining was used to display the general structure of the cervix.VG staining was applied to visualize the collagen fibers and muscular fibers.Immunohistochemistry was performed to observe the expressions of MMP-2 and MMP-8 in the cervix.Results HE staining showed that the rat uterine cervix consisted mainly of broblasts and brous connective tissues.A small quantity of neutrophils could be seen in the cervix stroma of the rats immediately after immediately parturition,but not at the other time points.The wet weight of the antepartum cervix had increased,and a more obvious increase was seen in the wet weight of the cervix immediately after parturition.The collagen fibers of the cervix consisted of collagen fibers and smooth muscle fibers,and their proportions showed no significant variation at the time points around the parturition.Immediately after parturition,the collagen fibers and muscular fibers in the cervix became loosened as compared with that before parturition.MMP-2 expression was found in the cervical stroma but not in the squamous epithelium in nonpregnancy,term pregnancy,and immediately after parturition;the smooth muscle cells,vascular wall,and stromal broblasts showed positive expression of MMP-2.Enhanced intensity of MMP-2 staining was seen in term pregnancy and postpartum group in comparison with that in the other groups.MMP-8 expression was observed in the cervix of rats immediately after parturition,with scattered neutrophils positive for MMP-8 spotted in the stroma of the ripened cervix.MMP-8 expression was not detected in the other groups.Conclusion Ripened cervical fibrous tissue becomes lo

关 键 词：基质金属蛋白2 基质金属蛋白8 大鼠 宫颈 

分 类 号：R714[医药卫生—妇产科学]