人参皂甙Rg3在体外对胃癌细胞生长和凋亡的影响  

Effects of ginsenoside Rg3 on growth and apoptosis of gastric cancer cell lines in vitro

作　　者：王吉[1] 史桂英[2] 袁耀宗[3] 乔敏敏[3] 章永平[3] 孙颖[1] 胡梅洁[3] 

机构地区：[1]上海交通大学医学院瑞金医院卢湾分院消化内科,上海200025 [2]上海交通大学基础医学院细胞生物学教研室,上海200025 [3]上海交通大学医学院瑞金医院消化内科,上海200025

出　　处：《上海交通大学学报（医学版）》2009年第11期1336-1340,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的研究人参皂甙Rg3在体外对低、中分化胃腺癌细胞株MKN-45和SGC-7901生长及凋亡的影响。方法取处于对数生长期的MKN-45和SGC-7901细胞,加入不同终浓度(20、30、40、50μg/mL)的人参皂甙Rg3分别培养24、48h或24、48和72h,以不加药细胞作为阴性对照。MTT法检测MKN-45和SGC-7901细胞生长抑制率;流式细胞仪AnnexinV/PI双染法检测SGC-7901细胞凋亡率;流式细胞仪分析SGC-7901细胞周期;透射电子显微镜观察50μg/mL人参皂甙Rg3处理组SGC-7901细胞的形态学改变。结果人参皂甙Rg3各处理组SGC-7901和MKN-45细胞生长抑制率均明显高于对照组(P<0.05),且随药物浓度的增加和作用时间的延长而上升(P<0.05)。与对照组比较,人参皂甙Rg3各处理组SGC-7901细胞凋亡率和G0/G1期细胞百分比均明显上升,且呈浓度和时间依赖性。透射电子显微镜观察50μg/mL人参皂甙Rg3处理组SGC-7901细胞,可见典型的凋亡细胞结构。结论人参皂甙Rg3在体外对胃癌细胞生长具有抑制作用,且呈现一定的时效和量效关系,其机制可能与诱导胃癌细胞凋亡有关。Objective To investigate the effects of ginsenoside Rg3 on growth and apoptosis of gastric cancer cell line MKN-45 and SGC-7901 in vitro. Methods MKN-45 and SGC-7901 cells at logarithmic growth phase were obtained,and were cultured with ginsenoside Rg3 of different concentrations （20,30,40,50 μg/mL）for 24,48 h or 24,48 and 72 h. Cells cultured without ginsenoside Rg3 were served as controls. The inhibition rates of ginsenoside Rg3 on MKN-45 and SGC-7901 cells were detected by MTT assay,apoptosis rate of SGC-7901 cells was determined by Annexin V/PI double staining flow cytometry,cell cycles of SGC-7901 cells were analysed by flow cytometry,and morphological changes of SGC-7901 cells in 50 μg/mL ginsenoside Rg3 treatment group were observed by transmission electron microscopy. Results The inhibition rates on MKN-45 and SGC-7901 cells in each ginsenoside Rg3 treatment group were significantly higher than those in control group （ P 0.05）,and the inhibition rates increased with the concentrations of ginsenoside Rg3 and time of culture （ P 0.05）. Compared with control group,the apoptosis rates of SGC-7901 cells and percentages of cells in G 0/G 1 cell cycle in each ginsenoside Rg3 treatment group were significantly increased in a concentration and time dependent manner. Typical morphology of SGC-7901 cell apoptosis was observed by transmission electron microscopy in 50 μg/mL ginsenoside Rg3 treatment group. Conclusion Ginsenoside Rg3 has significant inhibition effect on gastric cancer cell lines in vitro with a concentration and time dependent manner,the mechanism of which may involve the induction of gastric cell line apoptosis.

关 键 词：人参皂甙RG3 胃癌细胞株 细胞凋亡 细胞生长 抑制 

分 类 号：R285.5[医药卫生—中药学]