噁虫威原药毒性实验研究  

Study on Toxicity of Bendiocarb

作　　者：李华文[1] 陆丹[2] 吴军[2] 张丽娜[2] 杨秀鸿[2] 陈坚峰[2] 陈志莲[2] 

机构地区：[1]秦皇岛市疾病预防控制中心,河北秦皇岛066000 [2]湖南省劳动卫生职业病防治所,湖南长沙410007

出　　处：《工业卫生与职业病》2009年第6期332-335,共4页Industrial Health and Occupational Diseases

摘　　要：目的探讨噁虫威最大无作用剂量,为拟定人类接触该化学物的工作场所职业接触限值(OEL)提供基础资料。方法按照《化学品毒性鉴定技术规范》对噁虫威原药进行急性经口、经皮、眼刺激、皮肤刺激、皮肤致敏实验和大鼠亚慢性经口毒性实验。结果噁虫威原药雌、雄大鼠急性经口LD50均为31.6 mg/kg,属高毒类化学品;大鼠急性经皮LD50均>2150 mg/kg,属低毒类化学品;对实验兔眼睛和皮肤无刺激性;对白色豚鼠的致敏率为0%,属弱致敏类化学品。大鼠经口喂饲90 d染毒后,在中、高剂量水平,可引起雌鼠总蛋白、血清天门冬氨酸氨基转移酶、血肌酐、乳酸脱氢酶增高(P<0.05或P<0.01),雄鼠尿素氮明显增高(P<0.01);在高剂量水平,可引起雌鼠球蛋白、尿素氮明显增高(P<0.05或P<0.01),白球比明显降低(P<0.05),雄鼠血清天门冬氨酸氨基转移酶、血清丙氨酸氨基转移酶、血肌酐明显增高(P<0.05或P<0.01),全血胆碱酯酶活力明显降低(P<0.05)。结论该药属高毒类化学品,可能对肝脏、肾脏有损伤作用,在本实验条件下,噁虫威原药对雌、雄SD大鼠亚慢性(90 d)经口毒性实验的最大无作用剂量分别为0.10 mg/kg.d和0.09 mg/kg.d,OEL推算结果为0.0045 mg/m3,在制造、储运和使用过程中,须加强职业防护。Objective To study the toxicity of bendiocarb by acute oral and dermal toxicity tests in rats （LD50）, acute dermal and eye irritation tests in rabbits, dermal sensitization test in guinea pigs, and subchronic toxicity test in rats and to provide basic data for setting no observed adverse effect level（NOAEL）and occupational exposure limit （OEL）. Methods The tests were conducted in compliance with the Technical Guideline of Chemicals Toxicity Evaluation. Results The oral and dermal LD50 values to both females and males were 31.6 mg/kg and 〉 2150 mg/kg bendiocarb classified as high and low toxicity chemicals respectively. Bendiocarb had no-irritability to rabbits＇eyes and skin, and the skin-sensitization rate in guinea pigs was 0%. In the sub-chronic oral toxicity test, the female rats（0.50, 2.47 mg/（kg bw-d））showed increased total protein, serum aspartate aminotransferase, creatinine and LDH compared with the control group（P〈0.01）, and the male rats（0.44,2.18 mg/（kg bw.d））showed increased BUN（P〈0.01）. In the highest dosage groups（2.47 and 2.18 mg/（kg bw.d）for female and male respectively）, globulin and BUN in female rats increased significantly （P 〈 0.01 ）and A/G decreased （P 〈 0.05 ）, while aspartate aminotransferase, alanine aminotransferase and creatinine in male rats increased significantly（P〈0.01）and choline esterase decreased（P〈0.05）. Conclusions Bendiocarb may damage animal＇s liver. NOVELs for subchronic oral toxicity tests（90 days）in male and female rats of bendiocarb were 0.10 mg/（kg bw· d）and 0.09 mg/（kg bw · d） respectively under the experimental conditions of the study. The evaluated OEL was 0. 0045 mg/m3 , so we should enhance measures for preventing and controlling occupational poisoning in the work places.

关 键 词：噁虫威原药 急性毒性 亚慢性毒性 最大无作用剂量 职业接触限值 

分 类 号：R512.6[医药卫生—内科学] R446.11[医药卫生—临床医学]