HPLC法测定盐酸麻黄碱滴鼻液中盐酸麻黄素的含量  

Content determination of Ephedrine Hydrochloride Nasal Drop by HPLC

作　　者：张秀梅[1] 袁如彩[1] 

机构地区：[1]徐州医学院附属医院,江苏徐州221002

出　　处：《中国医药导报》2009年第34期44-45,共2页China Medical Herald

摘　　要：目的:建立高效液相法测定盐酸麻黄碱滴鼻液中盐酸麻黄素含量的方法。方法:采用WatersXTerraRP-18(4.6mm×150mm,5μm)色谱柱;流动相:甲醇-0.1%三乙胺-4%冰醋酸(58∶42∶1.7);流速:1.0ml/min,检测波长:256nm。结果:盐酸麻黄素在4.000～12.000μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为100.50%,RSD=1.00%(n=5)。结论:本方法简便、灵敏、准确,可用于盐酸麻黄碱滴鼻液的质量控制。Objective： To establish a method for the content determination of ephedrine hydrochloride in Ephedrine Hydrochloride Nasal Drop by HPLC. Methods： The Waters XTcrra RP-18 （4.6mm×150mm,5μm） column was used. The mobile phases consisted of methanol, 0.1% triethylamine and 4% glacial acetic acid （58：42：1.7）, the flow rate was 1.0 ml/min and the detection wavelength was at 256 nm. Results： The linear ranges of Diphenhydramine Hydrochloride was at 4.000-12.000 μg （r=0.999 9）, the average recovery was 100.50% with a RSD of 1.00% （n=5）. Conclusion： The method is fast, reliable, accurate and can be used in the quality control of ephedrine hydrochloride in Ephedrine Hydrochloride Nasal Drop.

关 键 词：高效液相色谱法 盐酸麻黄素 盐酸麻黄碱滴鼻液 含量测定 

分 类 号：R927.2[医药卫生—药学]