采用ISSR分子标记法鉴别道地药材化橘红  

Identification of Exocarpium Citri Grandis by inter-simple sequence repeat markers

作　　者：邓锋[1] 莫结丽[1] 陈浩桉[1] 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《广东药学院学报》2009年第5期455-458,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省中医药管理局资助项目(2060036);广东省科技计划项目(2007B031407003)

摘　　要：目的探索用ISSR分子标记方法在核酸分子水平上鉴别道地药材化橘红。方法从20条ISSR引物中筛选合适的引物对化橘红、橘子、柚子等共16个样品进行PCR扩增及电泳分析,寻找特征位点。结果有2条ISSR引物扩增出较为明显的多态性特征条带,可单独或联合应用鉴别化橘红。结论ISSR作为一种简便、可靠的分子标记方法,可用于化橘红的鉴别。Objective To identify Exocarpium Cirri Grondis by inter - simple sequence repeat markers. Methods Primers suitable for routine analysis were screened from 20 inter-simple sequence repeat primers, then, they were used in PCR and sepharose electrophoresis of 16 samples of Exocarpium Cirri Grandis, Citrus reticulata and Citrus grandis （ Linn. ） Osbeck. Results Two of the 20 primers amplified polymophic bands and suitable for the identification of Exocarpium Citri Grandis. Conclusion Inter-simple sequence repeat markers provide a quick, reliable molecular marker for identification of Exocarpium Cirri Grandis.

关 键 词：化橘红 ISSR 分子标记技术 

分 类 号：R282.5[医药卫生—中药学]