HPLC法同时测定壮骨关节丸中3种有效成分的含量  

Simultaneous quantification of three active compounds in Zhuangguguanjie pills by HPLC

作　　者：杨静[1] 谭沛 潘桂湘[1] 代志 郭锦明[1] 

机构地区：[1]天津中医药大学中医药研究院/天津市中药化学及分析重点实验室,天津300193 [2]三九医药股份有限公司,广东深圳518209

出　　处：《广东药学院学报》2009年第5期472-475,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：天津市科技创新专项资金项目(07FDZDSH01011);国家科技支撑计划项目(2007BAI47B01)

摘　　要：目的建立同时测定壮骨关节丸中淫羊藿苷、补骨脂素和异补骨脂素含量的HPLC方法。方法采用Zorbax SB-C18（4.6 mm×150 mm,5μm）色谱柱;流动相：乙腈-0.05%甲酸水溶液（梯度洗脱）;检测波长：0→8 min为270 nm,8→15 min为246 nm;流速：1.0 mL/min;柱温为35℃。结果淫羊藿苷、补骨脂素、异补骨脂素分别在0.0223-0.713μg（r=0.9997）、0.0272-0.869μg（r=0.9998）、0.0213-0.681μg（r=0.9998）范围内与峰面积呈良好的线性关系;平均回收率分别为101.1%（RSD=3.27%）、97.66%（RSD=2.46%）、99.65%（RSD=2.13%）。结论该方法简便可行、重现性好、可作为壮骨关节丸的质量控制方法。Objective To establish an HPLC - VWD method for simultaneous quantification of icariin, psoralen and isopsoralen in Zhuangguguanfie pills by using switching wavelength. Methods The separation was performed on a Zorbax SB - C18（4.6 mm × 150 mm, 5 μm） column using the gradient elution which consisted of acetonitrile and 0.05% formic acid aqueous solution at the flow rate of 1.0 mL/min. The detection wavelength was at 270 nm in the first 8 min, while changed to 246 nm at 8 - 15 min. Column temperature was fixed at 35 ℃. Results For icariin , psoralen and isopsoralen, the linear ranges were 0. 022 3 - 0. 713 μg（ r = 0. 999 7 ）, 0. 027 2 - 0. 869 μg （ r = 0. 999 8 ） and 0. 021 3 - 0. 681 μg （ r = 0. 999 8） , respectively, and their average recoveries were 101.1% （RSD = 3.27% ） , 97.66% （RSD = 2.46% ） and 99.65% （ RSD = 2. 13% ） , respectively. Conclusion The method could be used for the quality control of Zhuangguguanjie pills, which was available with a good reproducibility.

关 键 词：壮骨关节丸 淫羊藿苷 补骨脂素 异补骨脂素 梯度洗脱 

分 类 号：R286.0[医药卫生—中药学]