卟啉-荧光素二元化合物体外清除活性氧自由基及抑制氧化损伤的作用  

Scavenging effects of porphyrin-fluorescein hybrid on scavenging of reactive oxygen species and inhibition of oxidative damages in vitro

作　　者：陆家政[1] 杜一凡[1] 韦国锋[2] 李振中[2] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]广西右江民族医学院基础学院,广西百色533000

出　　处：《广东药学院学报》2009年第5期508-511,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东药学院博士启动基金项目(43540119)

摘　　要：目的研究卟啉-荧光素二元化合物体外清除活性氧自由基及抗氧化的作用。方法利用光照核黄素产生超氧阴离子自由基O2.-和Fenton反应产生羟自由基.OH,用分光光度法测定卟啉-荧光素二元化合物体外清除活性氧自由基的作用,用硫代巴比妥酸(TBA)分光光度法研究卟啉-荧光素对.OH诱发卵磷脂脂质过氧化和DNA氧化损伤的抑制作用。结果卟啉-荧光素二元化合物能有效清除活性氧自由基,对卵磷脂脂质过氧化和DNA氧化损伤有显著抑制作用。结论卟啉-荧光素二元化合物是一种良好的体外抗氧化剂。Objective Study the scavenging effects of porphyrin-fluorescein hybrid on scavenging of reactive oxygen species and inhibition of oxidative damages in vitro. Methods The scavenging effects of porphyrinfluorescein hybrid on reactive oxygen species ·OH and O2 generated by Fenton reaction and riboflavin photosensitization were investigated by the means of spectrophotometry. And the inhibition effects of porphyrin-fluorescein hybrid on lipid peroxidation in the presence of lecithin and oxidation damage of DNA chain induced by hydroxyl radical were observed by spectrophotometry with thiobarbituric acid. Results The results showed that porphyrin-fluorescein hybrid could scavenge reactive oxygen species efficiently. It could also significantly inhibit lipid peroxidation in the presence of lecithin and oxidation damage of DNA by hydroxyl radical. Conclusion The porphyrin-fluorescein hybrid was an effective antioxidant in vitro.

关 键 词：卟啉-荧光素 活性氧自由基 抗氧化 体外 

分 类 号：R96[医药卫生—药理学]