Ag@SiO_2纳米复合物金属增强荧光释放法检测葡萄糖  

The Release of Metal-Enhanced Fluorescence Ag@SiO_2 Nanocomposites for Glucose Detection

作　　者：夏晓东 易平贵[1] 于贤勇[1] 

机构地区：[1]湖南科技大学化学化工学院,湘潭411201

出　　处：《应用化学》2009年第12期1456-1460,共5页Chinese Journal of Applied Chemistry

基　　金：国家重点基础研究发展规划"九七三"项目子项目(2003CB716005);国家自然科学基金(20772027)资助项目

摘　　要：制备了Ag@SiO2纳米复合物,罗丹明B通过物理掺杂结合在SiO2壳层。由于金属增强荧光效应,罗丹明B的荧光增强到4.7倍。Ag核易被H2O2氧化,Ag核氧化后产生荧光增强释放效应。基于金属增强荧光释放建立了一种新型葡萄糖检测方法,采用交联法在罗丹明B掺杂的Ag@SiO2纳米复合物的SiO2壳层固定葡萄糖氧化酶。检测浓度范围为0.2~6.8 mmol/L,检测限可达0.06 mmol/L。由于H2O2氧化Ag核反应迅速,检测体系对葡萄糖的响应快。Ag@SiO2 and rhodamine-doped Ag@SiO2 nanocomposites were prepared.Rhodamine fluorescence intensity was enhanced by a factor of 4.7 due to metal-enhanced fluorescence.The Ag core was easily oxidized by H2O2,enhancing the fluorescence release of rhodamine.Glucose oxidase was immobilized on the layer of SiO2 shell by chemical cross linked cluster.A novel method to detect glucose was developed based on metal-enhanced fluorescence in a concentration range of 0.2-6.8 mmol/L with a detection limit of 0.06 mmol/L.Speedy detection response was obtained upon the fast oxidation of Ag core by H2O2.

关 键 词：Ag@SiO2 纳米复合物 金属增强荧光 金属增强荧光释放 葡萄糖检测 

分 类 号：O655[理学—分析化学]