离子对RP-HPLC法测定人血清中硫酸奈替米星的浓度  

Determination of Concentration of Netilmicin Sulfate in Human Serum by Ion-pair RP-HPLC

作　　者：吴洪文[1] 王亚洲[1] 李锦燊[1] 

机构地区：[1]广西医科大学第四附属医院,柳州市545005

出　　处：《中国药房》2009年第35期2742-2744,共3页China Pharmacy

基　　金：广西壮族自治区卫生厅科研课题(Z2007276)

摘　　要：目的:建立以离子对反相高效液相色谱法测定人血清中硫酸奈替米星浓度的方法。方法:色谱柱为LunaC18,流动相为乙腈-水(15:85,pH2.0,含10mmol.L-1庚烷基硫酸钠),流速为0.8mL.min-1,柱温为40℃,检测波长为205nm。结果:奈替米星血药浓度在2～20mg.L-1范围内线性关系良好(r=0.9976);方法回收率为93.66%～96.05%,日内、日间RSD均<10%。结论:本方法灵敏度较高,结果准确可靠,操作简便,适用于奈替米星血药浓度的临床监测。OBJECTIVE： To establish an ion pair RP - HPLC method for determination of the concentration of netilmicin sulfate in human serum. METHODS： The determination was performed on Luna C18 column with column temperature set at 40℃ ; the mobile phase consisted of aeetonitrile-water （15 ： 85, pH 2.0, containing l0 mmol· L-1 sodium heptanesulfonate） at a flow rate of 0.8 mL · min -1.The detection wavelength was set at 205 nm.RESULTS： The linear range of netilmicin was 2--20 mg · L -1（r = 0.997 6） with a recovery rate of 93.66%--96.05%. The intra- day and inter- day RSD were both less than 10%, respectively. CONCLUSION ： The method is sensitive and simple with accurate and reliable results, and it is able to satisfy the requirement for the determination of serum concentration of netilmicin.

关 键 词：反相高效液相色谱法 离子对 硫酸奈替米星 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R978.12[医药卫生—药学]