阿托伐他汀对血管内皮细胞增殖过程中FKBP12mRNA和细胞上清液ET-1的影响  

Effects of atorvastatin on mRNA expression of FKBP12 in vascular endothelial cell proliferation and the concentration of ET - 1 in supernatant fluid of endothelial cell

作　　者：王耀辉[1] 乔淑贞[1] 李小静[1] 

机构地区：[1]河南省平顶山市第一人民医院心内科,467000

出　　处：《中国实用医刊》2009年第24期23-25,共3页Chinese Journal of Practical Medicine

摘　　要：目的探讨不同浓度阿托伐他汀对血管内皮细胞（VEC）增殖过程中FK506结合蛋白12（FKBP12）表达和细胞上清液ET-1的影响。方法培养大鼠VEC并传代，观察其不同浓度阿托伐他汀作用下的不同时期FKBP12mRNA水平的表达和细胞上清液ET-1的变化。结果阿托伐他汀抑制血管内皮细胞增殖过程中FK—BP12的表达，抑制血管内皮细胞合成和分泌ET-1；血管内皮细胞FKBP12表达和ET-1分泌基本一致，呈正相关，阿托伐他汀对它们的抑制作用且呈时间依赖性和剂量依赖性。结论阿托伐他汀通过降低FKBP12表达抑制血管内皮细胞增殖并对ET-1合成和分泌起负性调节作用。Objective To evaluate the effect of atorvastatin on mRNA expression of FK506 binding protein 12 （FKBP12） in vascular endothelial cell（VEC） proliferation and the concentration of ET - 1 in supernatant fluid of endothelial cell. Methods Rat vascular endothelial cells were cultured with enzyme digesting methods, and were identified by measuring their growth rate with invertmicroscope, by using immuocytochemical methods with factor Ⅷ antibody. Then the cells were treated with atorvastatin in different concentration. The proliferation of rat vascular endothelial cell was observed under an invert microscope. The expression of FKBP12 mRNA and the concentration of ET - 1 in supernatant fluid of endo- thelial cell were detected in different culture periods. Results Atorvastatin markedly restrained the expressions of FKBP12 mRNA in VEC and the concentration of ET - 1 in supernatant fluid of endothelial cell. A significantly positive correlation of them was indentified. The effect appeared dose dependence and time dependence. Conclusions Atorvastatin dose - dependently and time - dependently restrained the proliferation of rat VEC. As a result, the expression of FKBP12 mRNA and the concentration of ET - 1 in supernatant fluid of endothelial cell are decreased too.

关 键 词：阿托伐他汀 血管内皮细胞 增殖 FK506结合蛋白12 

分 类 号：R9[医药卫生—药学]