输血与血型不规则抗体检测的关系  

Relationship between transfusion and detection of blood type irregular antibodies

作　　者：陆紫敏[1] 祖亚钧[1] 梁萍[1] 纪黎明[1] 孙向华[1] 

机构地区：[1]同济大学附属同济医院输血科,上海200065

出　　处：《临床血液学杂志（输血与检验）》2009年第6期635-637,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:探讨输血与血型不规则抗体检测的关系。方法:用卡式凝胶法对4790例输血患者的备血样本进行血型不规则抗体检测并进行输血史调查;阳性标本加传统的试管抗人球蛋白法做比较。结果:检出不规则抗体总阳性率为0.71%,其中自身抗体6例,同种特异性抗体26例;3次以上输血组与3次以下输血组比较检出不规则抗体阳性率差异有统计学意义(P<0.05),卡式凝胶法检出抗体阳性率与传统的试管抗人球蛋白法比较差异有统计学意义(P<0.05)。结论:不规则抗体产生与输血频率关系密切;输血前作不规则抗体检测和鉴定在保障临床手术或药物治疗及安全有效输血中具有重要临床意义;选用卡式凝胶法检测不规则抗体,其灵敏度高,结果可靠,操作简便,值得临床推广常规应用。Objective：To investigate the relationship between transfusion and detection of blood type irregular antibodies. Method：Blood type irregular antibodies of 4 790 patients were detected by Microcolumn Gel Coombs test（MGCT） and their histories of blood transfusion were analyzed. Positive specimens were detected by tradition al Coombs method again to compare. Result：Total positive rate was 0.71 %, including 6 cases with autoantibodies and 26 cases with specific alloantibodies. The positive rate of more than 3 times transfusion group was significantly higher than that of less than 3 times transfusion group （ P〈0.05） ;The positive rate of MGCT was significantly higher than that of traditional Coombs test （ P 〈0.05）. Conclusion：The occurrence of irregular antibodies is relat ed with the frequency of transfusion. It is meaningful to perform irregular antibody screening and identification to ensure safety and effectiveness treatment of clinical surgery or drug and hlood transfusion. Moreover, MGCT, for irregular antibody screening, is a sensitive, accurate, convenient, specific and reliable, which is worthy applying in the clinical practice.

关 键 词：不规则抗体 微柱凝胶 输血反应 

分 类 号：R457.13[医药卫生—治疗学]