中缅边境西段恶性疟原虫对7种抗疟药敏感性的监测  

Surveillance of Plasmodium falciparum susceptibility to seven antimalarials,including artemether,in the western part of the Sino-Myanmar border area

作　　者：杨恒林[1] 李兴亮[1] 高白荷[1] 杨品芳[1] 张志勇[1] 

机构地区：[1]云南省寄生虫病防治所,云南省疟疾研究中心,云南普洱665000

出　　处：《中国病原生物学杂志》2009年第11期831-832,843,共3页Journal of Pathogen Biology

基　　金：世界卫生组织西太区办公室项目(WPR02048)

摘　　要：目的了解中缅边境西段恶性疟原虫对蒿甲醚等7种抗疟药的敏感性,以指导合理用药。方法2003年采用WHO推荐的体外微量法测定恶性疟原虫对蒿甲醚、氯喹、哌喹、萘酚喹、咯萘啶、本芴醇和青蒿琥酯7种抗疟药的敏感性。结果对恶性疟原虫的ID50和CI MC,蒿甲醚为299.2和1 253.2 nmol/L;氯喹为123.0和325.9 nmol/L,抗性率83.33%;哌喹为167.5和628.1 nmol/L,抗性率91.67%;萘酚喹为57.5和176.6 nmol/L;本芴醇为89.5和472.1nmol/L;咯萘啶为40.1和137.1 nmol/L,抗性率31.58%;青蒿琥酯为14.4和30.6 nmol/L。结论当地恶性疟原虫对氯喹、哌喹仍具抗性,但抗性程度呈下降趋势;对青蒿素类药物及咯萘啶基本敏感,但敏感性呈下降趋势;对新药萘酚喹、本芴醇敏感。Objective To ascertain the susceptibility of Plasmodium falciparum to seven antimalarials,including artemether,in the Western part of the Sino-Myanmar border area and to guide rational antimalarial use.Methods An in vitro micro-test recommended by the WHO was used to assay the susceptibility of P.falciparum to artemether,chloroquine,piperaquine,pyronaridine,naphthoquione,benflumetol,and artesunate in 2003.Results With respect to P.falciparum,the ID50 and CIMC（mean concentration completely inhibiting schizont formation） of artemether were 299.2 and 1 253.2 nmol/L and those of chloroquine were 123.0 and 325.9 nmol/L, with a resistance rate of 83.33%. The ID50 and CIMC of piperaquine were 167.5 and 628.1 nmol/L, and the resistance rate was 91.67%. The ID50 and CIMC of pyronaridine were 40.1 and 137.1 nmol/L, and the resistance rate was 31.58%. The ID50 and CIMC of benflumetol were 34.1 and 494.0 nmol/L. The ID50 and CIMC of naphthoquione m were 57.5 and 176.6 nmol/L. The ID50 and CIMC of artesunate were 14.4 and 30.6 nmol/L. Conclusion Local P. falciparum still has resistance to chloroquine and piperaquine, but its susceptibility has tended to decrease. It is susceptible to malaridine and artemisinin-based drugs, but its susceptibility has tended to decrease. P. falciparum is susceptible to the new antimalarials naphthoquione and benflumetol.

关 键 词：疟原虫 恶性 抗疟药 药物敏感性 体外微量法 

分 类 号：R382.31[医药卫生—医学寄生虫学]