高效液相色谱法测定洗发产品中5种违禁添加药物  

Determination of five prohibited drugs in hair shampoo products by HPLC

作　　者：徐春祥[1] 仓义鹏 车文军[1] 徐楠楠[1] 

机构地区：[1]江苏省产品质量监督检验研究院国家化妆品质量监督检验中心,江苏南京210029 [2]宿迁市产品质量监督检验所,江苏宿迁223800

出　　处：《日用化学工业》2009年第6期442-444,共3页China Surfactant Detergent & Cosmetics

基　　金：国家质检总局质量技术监督科技计划资助项目(2005QK119)

摘　　要：建立了高效液相色谱法同时测定洗发产品中酮康唑、甲硝唑、氯霉素、丙酸氯倍他索及醋酸曲安奈德等5种违禁药物成分的方法。在SunF ire-C18(5μm,4.6 mm×150 mm)色谱柱上,以甲醇和乙酸铵水溶液梯度洗脱,检测波长为254 nm,流速为1 mL/m in,柱温为30℃。检出限为0.08 mg/L^0.20 mg/L,各组分在2.0 mg/L^50.0 mg/L呈良好线性关系,相关系数为0.999 5~0.999 9,样品加标回收率为89.2%~97.8%,相对标准偏差为1.56%~3.26%。A HPLC method was established for the determination the content of ketoconazole,metronidazole,chloramphenicol,clobetasol propionate and triamcinolone acetonide acetate in hair shampoo products on a SunFire-C18（5 μm,4.6 mm×150 mm） column using methanol/ammonium acetate aqueous solution for gradient elution.Flow rate of the mobile phase is 1 mL/min.Column temperature is 30 ℃ and detection wavelength is 254 nm.Detection limit of this method is 0.08 mg/L-0.20 mg/L.The results showed that as the concentration of the above mentioned ingredients is in a range of 2.0 mg/L～50.0 mg/L,there is good linearity between the peak area and the concentration.The correlation coefficient is 0.999 5-0.999 9.The average recovery of shampoo sample is 89.2%-97.8% and the relative standard deviation is 1.56%-3.26%.

关 键 词：洗发产品 违禁药物 高效液相色谱法 

分 类 号：TQ658.3[化学工程—精细化工]