Dimension RxL MAX全自动生化分析仪血清铁测定系统的建立  

Establishment of a serum iron assay system on Demension RxL Max automatic biochemical analyzer

作　　者：陈敏[1] 易浔飞[1] 廖剑[1] 

机构地区：[1]南京军区福州总医院全军检验医学研究所,福州350025

出　　处：《分析仪器》2009年第6期26-29,共4页Analytical Instrumentation

摘　　要：在Dimension RxL Max全自动生化分析仪的开放通道上用空白试剂盒装载商品化亚铁嗪检测试剂,建立了血清铁的测定体系,并对所建方法进行了方法学评价。结果表明,该方法的高、低值样本总变异系数分别为5.55%、7.49%,测定线性范围为2～1000μg/dL,平均回收率为104%。实验证明,在该仪器开放通道中运`用亚铁嗪法进行血清铁测定是可行的。Objective： To evaluate methodologically the serum iron assay system establised on Demension RxL Max automatic biochemical analyzer. Methods： The blank reagent cartridge was filled with commercial re- agents and the assay parameters were programmed on the open channel. The precision and linearity of the method were evaluated using the guidelines of NCCLS EP5A and EP6A, respectively. The test results of this method were compared with those of commercial method using serum samples of patients. Results： The linear range is 2-1000 μg/dL, the total CVs for high value and low value samples are 5.55% and 7. 49 %, respectively. The average recovery is 104%, and the regression equation is y = 0. 9691x-3. 6437 （r = 0. 9890） . Conclusion： The method is sensitive, accurate, and feasible for serum ion level monitoring.

关 键 词：DIMENSION RxL Max全自动生化分析仪 血清铁 亚铁嗪 

分 类 号：TH776[机械工程—仪器科学与技术]