正常皮肤、增生性瘢痕和瘢痕疙瘩成纤维细胞培养、生物学特征及超微结构的比较研究  

Comparative study on biological characteristics and ultrastructure of the fibroblasts derived from normal skin, hypertrophic scar and keloidin in vitro culture

作　　者：宋维铭[1] 孙广慈[1] 管正玉[1] 徐承熊[2] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院,北京100041 [2]中国医学科学院药物研究所

出　　处：《中华整形烧伤外科杂志》1998年第6期410-413,共4页

基　　金：卫生部科研基金资助

摘　　要：目的探讨正常皮肤、增生性瘢痕和瘢痕疙瘩成纤维细胞的体外培养、生物学特征及超微结构,阐明其应用价值。方法采用成纤维细胞体外培养技术,从正常皮肤成纤维细胞与增生性瘢痕、瘢痕疙瘩成纤维细胞在细胞增殖、细胞形态、细胞遗传学特征及细胞超微结构方面进行比较研究。结果体外培养的正常皮肤成纤维细胞与增生性瘢痕、瘢痕疙瘩成纤维细胞生长率和形态学相同,细胞遗传学特征和超微结构相似。结论据此结果和其他学者的观点,认为建立体外培养的正常皮肤成纤维细胞的细胞实验模型,可用于瘢痕防治的研究。Objective This study was to investigate the in vitro cultivation, biological character- istics and ultrasturcture of the fibroblasts derived from normal skin, hypertrophic scar and keloid, to elu- cidate the value of their application. Method A comparative study was performed on cell proliferation, cell morphology, cytogenetic feature and cell ultrastructure of fibroblasts from normal skin, hypertrophic scar and keloid by means of the technique of in vitro culture. Result The results indicated that the fi- broblasts isolated from normal skin, hypertrophic scar and keloid exhibited similar morphology and growth rates. The cytogenetic feature and cell ultrastructure were also alike. Conclusion According to our study and that of others, we conclude that it is entirely feasible to establish an in vitro culture model of fibroblasts from normal skin for investigation, prevention and treatment of scars.

关 键 词：增生性瘢痕 瘢痕疙瘩 成纤维细胞 超微结构 

分 类 号：R619.202[医药卫生—外科学]