肝缺血再灌注损伤和ATP-MgCl_2含氧晶体灌注液的保护作用——肝细胞病理变化观察  

Hepatic ischemia reperfusion injury and protective effects with infusion of ATP MgCl 2 and oxygenated crystalloid solution and the observation of hepatocellular pathologic changes

作　　者：庞志刚[1] 王广田[1] 黄冬梅[1] 孙培春[1] 赵月春[1] 郑蔚[1] 

机构地区：[1]河南医科大学第二附属医院

出　　处：《中国老年学杂志》1998年第5期284-286,共3页Chinese Journal of Gerontology

基　　金：河南省卫生厅重点项目

摘　　要：取健康大耳白兔１６只，体重２．１～２．４ｋｇ，随机分为２组。Ⅰ组：肝门阻断后，通过门静脉向肝内持续灌注生理盐水（４０～４５ｍｌ／ｋｇ·ｈ－１）；Ⅱ组灌注ＡＴＰ－ＭｇＣｌ２（１００μｍｏｌ／ｋｇ·ｈ－１）＋含氧生理盐水（４０～４５ｍｌ／ｋｇ·ｈ－１）。阻断６０ｍｉｎ后恢复肝血流灌注１２０ｍｉｎ。于缺血前、缺血后６０ｍｉｎ及再灌注１２０ｍｉｎ分别切取肝组织行光镜及电镜检查。结果显示，光镜下及电镜下改变：Ⅱ组缺血６０ｍｉｎ及再灌注１２０ｍｉｎ细胞损伤程度均较Ⅰ组同期明显减轻。认为ＡＴＰ－ＭｇＣｌ２含氧晶体灌注液能有效防止肝缺血再灌注损伤，显著提高肝细胞对缺血的耐受能力。methods Sixteen white rabbits(2.1～2.4 kg) were randomly divided into two groups. After occlusion, the liver was constantly perfused through the potal vein for 60 min with different perfusates. Group Ⅰ and Group Ⅱ received normal saline(40～45 ml/kg·h -1 ) and ATP MgCl 2(100 μmol/kg·h -1 )+oxygenated normal saline(40～45 ml/kg·h -1 ) respectively. After 60 min of occlusion, the blood reperfusion was continued for 120 min. Hepatic tissue samples were obtained just before ischemia, 60 min after occlusion and 120 min after reperfusion. The specimen were examined under light and electronic microscope. Results Light and electronic microscopy revealed that pathologic changes were more severe after 60 min of ischemia and after 120 min of reperfusion in GroupⅠ, but these changs were slightest in GroupⅡ.Conclusion Adiministration of ATP MgCl 2+oxyenated normal saline through potal vein during early stage of hepatic ischemia can effectively prevent hepatic ischemia reperfusion injury and improve the toleranece of the hepatocellular ischemia.

关 键 词：ATP MGCL2 含氧晶体液 再灌注损伤 病理 肝缺血 

分 类 号：R657.3[医药卫生—外科学]