PAI-1基因多态性与2型糖尿病的相关性  

Relationship between the gene polymorphism of PAI-1 and type 2 diabetes mellitus

作　　者：辛萍[1] 申黎艳[2] 郑春喜 姜靖[1] 隋菊英 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]青岛市市立医院 [3]青岛市商业职工医院 [4]莱阳市康复医院

出　　处：《山东医药》2009年第43期19-21,共3页Shandong Medical Journal

基　　金：青岛市科技局科技发展资助项目(05-1-NS-77)

摘　　要：目的分析2型糖尿病患者(T2DM)纤溶酶原激活物抑制物(PAI)-1基因型及分布频率,探讨PAI-1基因多态性与T2DM的关系。方法观察组为34例T2DM患者,对照组为39例健康体检正常者,采用等位基因特异性引物PCR扩增技术检测各组人群PAI-1基因多态性,两组人群血浆PAI-1含量检测采用美国USCNLIFE原装人PAI-1 ELISA试剂盒,酶标仪450 nm波长下测定吸光度(OD值),两次平行测定取均值计算样品PAI-1水平。结果观察组与对照组相比PAI-1基因型频率及等位基因频率均有显著性差异(χ2=24.127,P<0.001;χ2=7.312,P<0.05)。观察组4G等位基因频率明显高于对照组,有显著性差异(χ2=6.280,P<0.05)。观察组血浆PAI-1水平明显高于对照组(t=10.7,P<0.01),观察组内比较4G/4G者血浆PAI-1含量明显高于4G/5G和5G/5G者,有显著性差异(F=21.02,P<0.001)。结论PAI-1基因多态性与T2DM间存在一定的关联性,其中4G等位基因可能是T2DM发病的危险因子。Objective To analyze the gene type and distribution frequency of plasminogen activator inhibitor type-1 （PAI-1） in patients with type 2 diabetes mellitus （T2DM）, and to explore the relationship between the gene polymorphism of PAI-1 and T2DM. Methods Thirty-four patients with T2DM （observed group） and 39 healthy controls （control group） were enrolled in the study. The gene polymorphism of PAI-1 in two groups was detected by specific polymerase chain reaction （PCR）, and plasma level of PAI-1 was measured by ELISA. Results There were significant differences in PAI-1 gene types frequency and allele frequency between the two groups （X^2 = 24. 127, P 〈 0. 001 ;X^2 = 7.312, P 〈 0.05 ）. The frequency of 4G allele frequency in the observed group was significantly higher than that in the control group （X^2 = 6. 280 ,P 〈 0.05 ）. The plasma level of PAl-1 in the observed group was significantly higher than that in the control group（ t = 10.7 ,P 〈 0.01 ）, and which in the patients with gene type of 4G/4G was significantly higher than that in the patients with gene type of 4（;/5 G 和 5G/5G（ F = 21.02 ,P 〈 0.001 ）. Conclusion There lies a recorrelation between the gene polymorphism and T2DM, the 4G allele of PAI-1 maybe a risk factor for the generation of T2DM.

关 键 词：纤溶酶原激活抑制物-1 基因多态性 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]