胎儿软骨中环丙沙星药物浓度RP—HPLC测定法  

RP-HPLC Assay for Ciprofloxacin Concentration in Foetus Cartilagines

作　　者：孙效东[1] 曲芬[2] 

机构地区：[1]解放军总医院临床药理研究室,北京100853 [2]北京302医院,100039

出　　处：《药物分析杂志》1998年第6期380-383,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：采用反相高效液相色谱法建立了测定胎儿软骨中环丙沙星药物浓度的方法 ,实验采用WatersHPLC系统 ,μBondapakC18柱 ,以乙腈— 0 0 2mol/L磷酸盐缓冲液—三乙胺 (30∶70∶0 0 5 )为流动相 ,内标法定量 ,在λ=2 77nm进行检测。在 31 2 5～ 2 0 0 0 μg/mL范围内环丙沙星和内标峰高比与软骨样本环丙沙星浓度呈良好线性关系 (r=0 9990 ) ,方法回收率为 99 3%～ 10 3 2 % ,日内、日间RSD分别为 1 9%～ 5 8%、 2 4 %～ 7 2 % ,n=5 。用此法测定了Osteochondrodysplasia fetalis is one of adverse reactions of quinolones.To determine quinolones concentration in cartilagines is a important means for investigating the mechanism of the disease.A sensitive and precise RP-HPLC method for determination of ciprofloxacin in foetus cartilagines was developed.Waters HPLC system and Waters μ Bondapak C 18 column and phosphate buffer-acetonitrile-triethylamine as a mobile phase was used.Methodology evaluation shown that ciprofloxacin cartilage concentration between 31 25～2000 μg/L had a good linear coefficient relation(r=0 9990).The detection wavelength was set at 277 nm.The average recovery of the method was 99 3 %～103 2 %.The RSD of within-day and between-days were 1 9 %～5 8 % and 2 4 %～7 2 %.Ciprofloxacin cartilagines concentrations of 10 induced labor foetus were determined with the method.ryofthemetho

关 键 词：胎儿 软骨 HPLC 环丙沙星 含量测定 

分 类 号：R978.19[医药卫生—药品] R927.2[医药卫生—药学]