RP—HPLC法测定头孢克罗分散片的含量  

Determination of Cefaclor Dispersible Tablets by RP-HPLC

作　　者：周志凌[1] 

机构地区：[1]安徽省药品检验所,合肥230061

出　　处：《药物分析杂志》1998年第6期386-388,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：报道了头孢克罗分散片中头孢克罗的反相高效液相色谱法 ,采用ODS柱 (2 5 0mm×4 6mm) ,水— 3 86 %醋酸钠溶液—甲醇 (1380∶2 9∶5 91)为流动相 ,乙酰苯胺为内标 ,用紫外检测器于 2 5 4nm波长处检测。平均回收率为 99 83% ,RSD =0 72 % (n=5 ) ,线性范围为 0 1～ 0 5mg/mL (r=0 9996 )。检测限为 1 6× 10 -4μg。本法具有快速、简便、灵敏。A RP-HPLC method was established for the determination of cefaclor in its dispersible tablets with acetanilid as internal standard.Cefaclor can be separated on ODS column with a mixture of water,3 86 % sodium acetate solution and methyl alcohol(1380∶29∶591)as mobile phase and detected at 254 nm.The flow rate was 0 8 mL/min.The standard curve was linear in the concentration range of 0 1 to 0 5 mg/mL and the correlation coefficient was 0 9996.The average recovery and relative standard deviation were 99 83 % and 0 72 % respectively.The detection limit was 1 6×10 -4 μg.The method is simple,rapid,accurate and reliable.

关 键 词：高效液相色谱 头孢克罗 分散片 含量测定 

分 类 号：R978.11[医药卫生—药品] R927.2[医药卫生—药学]