日本血吸虫童虫培养细胞SOD和MDA动态变化的研究  

STUDIES ON DYNAMIC CHANGES OF SOD AND MDA IN CULTURED CELLS FROM SCHISTOSOMA JAPONICUM SCHISTOSOMULA

作　　者：蒋明森[1,2] 陈晓蓓[1,2] 董惠芬[1,2] 杨明义 倪永晖[1,2] 周述龙 

机构地区：[1]湖北医科大学寄生虫学教研室 [2]湖北医科大学附属第一医院传染科

出　　处：《中国寄生虫学与寄生虫病杂志》1998年第5期357-359,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：湖北省教委和卫生厅资助

摘　　要：目的：观察日本血吸虫童虫细胞在体外培养过程中超氧化物歧化酶（ＳＯＤ）和丙二醛（ＭＤＡ）含量的动态变化，探讨培养细胞的退化过程。方法：应用邻苯三酚自氧化法及硫代巴比妥酸法分别测定童虫细胞在培养０－５４ｄ过程中ＳＯＤ和ＭＤＡ含量的变化。结果：在培养第１２ｄＳＯＤ出现一个高峰，以后一直处于低平状态。ＭＤＡ在培养第１８ｄ和第４８ｄ分别各有一个高峰，其后均逐渐降低。结论：日本血吸虫童虫培养细胞呈逐步退化过程。AIM:To observe the dynamic changes of superoxide dismutase(SOD) and maldondialdehyde(MDA) in cultured cells from Schistosoma japonicum schistosomula and to explore the degeneration process of cultured cells. METHODS: Pyrogallol method and thiobarbituric acid method were used to determinate the levels of SOD and MDA , respectively,in the cells cultured for 0-54 days. RESULTS: SOD in the cells reached a peak level on d12 of the early stage of culture and then decreased . The MDA levels in the cultures cells ascended gradually, and had two peaks on d18 and d48, during the period of culture. CONCLUSION:The cultured cells from S. japonicum schistosomula degenerated progressively.

关 键 词：日本血吸虫 童虫 培养细胞 SOD MDA 

分 类 号：R383.24[医药卫生—医学寄生虫学]