HPV18-E2与IL-12联合基因疫苗免疫效果研究  

Study on Immune Response Induced by HPV18-E2 DNA Vaccine Combined with IL-12

作　　者：阳帆[1] 张琴[1] 朱翠明[1] 陈苏芳[1] 陈杰[1] 万艳平[1] 

机构地区：[1]南华大学病原生物学研究所,湖南衡阳421001

出　　处：《实用预防医学》2009年第6期1698-1701,共4页Practical Preventive Medicine

基　　金：湖南省科学技术厅计划项目(2009FJ3048)

摘　　要：目的研究人乳头瘤病毒18型E2基因（HPVl8-E2）疫苗的免疫效果及白细胞介素-12（IL-12）对其免疫作用的影响。方法选6～8周龄Balb／c雌性小鼠35只，随机分为5组，即PBS组、pcDNA3．1（＋）空质粒组、pcD．NA3．1（＋）／E2组、pcDNA3．1（＋）／IL-12组和pcDNA3．1（＋）／E2＋pcDNA3．1（＋）／IL-12组，每组7只。小鼠肌肉注射DNA疫苗，200ug/次。隔2周免疫1次，共免疫4次。于0、2、4、6周剪尾取血，第8周摘眼球放血。ELISA测血清中的特异性IgG抗体及小鼠脾淋巴细胞培养上清IFN-γ、IL-4，MTT比色法检测淋巴细胞的增殖。结果免疫8周pcD—NA3．1（＋）／E2及pcDNA3．1（＋）／E2＋pcDNA3．1（＋）／IL-12疫苗组抗体IgGA值显著升高，与对照组比较差异有统计学意义（P〈0．01），pcDNA3．1（＋）／E2及pcDNA3．1（＋）／E2＋pcDNA3．1（＋）／IL-12组小鼠脾淋巴细胞培养上清IFN-γ、IL-4水平明显高于其他对照组，差异有统计学意义（P〈0．01），pcDNA3．1（＋）／E2＋pcDNA3．1（＋）／IL-12组小鼠脾淋巴细胞增殖活性明显增强，与pcDNA3．1（＋）／E2组比较，差异无统计学意义（P〉0．05），与其他对照组比较差异有统计学意义（P〈0．01）。结论pcDNA3．1（＋）／E2＋pcDNA3．1（＋）／IL-12核酸疫苗联合免疫小鼠能够有效的诱导细胞免疫和体液免疫应答，且免疫效应比pcDNA3．1（＋）／E2基因疫苗强。Objective To study the effectiveness of human papillomavirus type 18 E2 gene （HPV18- E2） vaccine and interleukin- 12（IL - 12） on their immune effects. Methods Thirty- five Balb/c female mice aged 6 to 8 - week - old were randomly divided into five groups, namely, PBS group, pcDNA3.1 （ ＋ ） empty plasmid group, pcDNA3.1 （ ＋ ） / E2 group, pcDNA3. 1 （＋） / IL-12 group, andpcDNA3.1 （＋） / E2 ＋ pcDNA3.1 （＋） / IL-12 group （eachn=7）. The mice were given intramuscular injection of DNA vaccines at 2 - week interval for four times, and 100mg/times. The tails were cut to draw blood at the 0, 2, 4, 6 weeks, and then the mice were executed by eyeball blood letting at the 8 weeks. ELISA was used for the quantitative detection of the specific IgG antibody in the sera of Balb/c mice and the cytokine IFN - γ and IL4 in mice spleen lymphocyte culture medium after stimulating by HPVl8 - E2. The proliferation response of spleen cells was detected by MTT assay. Results After 8 weeks immunization, antibody IgG A in pcDNA3.1 （ ＋ ） / E2 vaccine group and pcDNA3.1 （ ＋ ） / E2 ＋ pcDNA3.1 （ ＋ ） / IL - 12 vaccine group was significantly increased as compared with that of control group, and the difference was statistically significant （P〈 0.01）. IFN- γ and IL- 4 levels of mice spleen lymphocyte culture supernatant in pcDNA3.1 （ ＋ ） / E2 group and pcDNA3.1 （ ＋ ） / E2 ＋ pcDNA3.1 （ ＋ ） / IL- 12 group were significantly higher than those of the other control groups, and the difference was statistically significant （P〈 0.01）. The spleen lymphopoiesis activeness of pcDNA3.1（ ＋ ）/E2 ＋ pcDNA3.1（ ＋ ）/IL- 12 group was significantly enhanced as compared with the pcDNA3.1（ ＋ ）/E2 group, no statistically significant difference was found between them（P〉0.05）, but as compared with the other control groups, there were statistically significant differences （P 〈 0.01）. Conclusions pcDNA3.1 （＋ ） / E2 ＋ pcDNA3.1 （ ＋ ） / IL-

关 键 词：HPV18 E2 IL-12 疫苗 

分 类 号：R392-33[医药卫生—免疫学]