用PCR-SSP法分析中国辽宁汉族HLA-DRBI基因多态性  

The polymophic study of HLA-DRB1 gene in Chinese Liaoning Han with PCR-Sequence specific primers

作　　者：刘利民[1] 胡姝[1] 贾静涛[1] 

机构地区：[1]中国医科大学法医系血清学教研室,辽宁沈阳110001

出　　处：《法医学杂志》1998年第4期204-206,共3页Journal of Forensic Medicine

基　　金：国家教委留学归国人员科研基金!95135;辽宁省科委自然科学基金!962312

摘　　要：应用PCR－SSP方法对辽宁地区159名无关个体进行HLA－DRB1位点基因分型，检出8组等位基因（扩增片段大小为100bp），基因频率范围在0．02201～0．23899。36种可能基因型中检出33种。经x2检验符合Hardy－Weinberg平衡定律。本地区汉族群体的期望杂合度为85％，观察杂合度为83％。个人鉴别机率（DP）为0．94非父排除率（EPP）为66％。本法具有简单、快速、结果可靠的特点，不仅适用于法医学亲子鉴定和个人识别、移植配型，亦可用于相关疾病及人类遗传学研究。8 allele and group specificity at HLA - DRB1 locuswere genotyped in 159 Unrelated individuals of Liaoning Han,with polymerase chain reaction - sequence specific primers.The range of gene frequencies was from 0 .02201 - 0 .23899.Heterozagosity was 83%. 33 out of 36 theoretical genotypeswere identified and the result conformed to Hardy- Weinbergequilibrium law. The present method can be used not only inpaternity test, individual identification, but also in clinicgraft matching between donor and recipient as well as thestudy of HLA.

关 键 词：HLA DRB1基因 PCR 基因频率 基因多态性 

分 类 号：D919.4[医药卫生—法医学] Q346.5[政治法律—法学]