草乌炮制前后HPLC指纹图谱比较研究  

Study on HPLC Fingerprint between Radix Aconiti Usnezoffii and Radix Aconiti Kusnezoffii Preparata

作　　者：于永军[1] 李俊松[2] 蔡皓[2] 杨光明[2] 蔡宝昌[2] 

机构地区：[1]沧州医学高等专科学校,河北沧州061001 [2]南京中医药大学,江苏南京210029

出　　处：《现代中药研究与实践》2009年第6期46-48,共3页Research and Practice on Chinese Medicines

摘　　要：目的采用HPLC方法研究草乌炮制前后HPLC指纹图谱变化。方法以kromasil-C18色谱柱为固定相,乙腈:缓冲液(0.2%冰醋酸用三乙胺调节pH至6.20)为流动相进行梯度洗脱。结果草乌炮制前后指纹图谱中主要色谱峰发生了显著改变。结论本研究建立了条件适中、重现性好的HPLC指纹图谱方法,可用于草乌及制品的质量控制。Objective To compare the difference of fingerprint between Radix Aconiti Usnezoffii and Radix Aconiti Kusnezoffii Preparata by HPLC. Methods Using kromasil-C18 chromatographic column as the fixed phase, with acetonitrile-buffer（0.2% glacial acetic acid adjust pH with triethylamine to 6.20） as mobile phase gradient elution. Results There were obvious difference in the fingerprint between Radix Aconiti Usnezoffii and Radix Aconiti Kusnezoffii Preparata. Conclusion The method is appropriate,reproducible and accurate,it can be used for controlling the quality of Radix Aconiti Usnezoffii and Radix Aconiti Kusnezoffii Preparata.

关 键 词：草乌 制草乌 高效液相色谱指纹图谱 

分 类 号：R283.3[医药卫生—中药学]