苏芸金芽孢杆菌的肠毒素及其编码基因的研究  

Study of the bceT and hblA genes and the hemolysin BL of Bacillus thuringiensis group

作　　者：张文成[1] 任改新[1] 

机构地区：[1]南开大学生物系

出　　处：《中华微生物学和免疫学杂志》1998年第6期428-433,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金

摘　　要：目的对苏芸金芽孢杆菌（简称Ｂｔ）种群不同亚种存在的蜡状芽孢杆菌肠毒素基因及溶血素ＢＬ进行检测，以便评估Ｂｔ对人类及高等动物潜在的致病性。方法采用多重引物ＰＣＲ技术，在同一ＰＣＲ体系同时加两对引物，ｈｂｌＡ及ｂｃｅＴ基因的扩增片段分别为１０１７ｂｐ及４２７ｂｐ，与预期的完全一致；应用ＨＢＬ羊血平板，产溶血素ＢＬ的菌株可产生非连续型溶血环。结果４５株Ｂｔ标准株中，２９株含有ｈｂｌＡ基因，占６４．４％；１５个生产用菌株及野生株中，含ｈｂｌＡ基因的菌株为１０株，占６６．６６％；含ｂｃｅＴ基因的标准株２９株，占６４．４％，含ｂｃｅＴ的Ｂｔ生产用菌株及野生株为１３株，占８６．６％。ＨＢＬ羊血平板检测结果表明：２８．８％的Ｂｔ标准株及３３．３％的Ｂｔ生产用菌株及野生株含溶血素ＢＬ。结论ｈｂｌＡ及ｂｃｅＴ基因在苏芸金芽孢杆菌中是普遍存在的，建议在遴选Ｂｔ生产菌株时。Objective The hemolysin BL and the genes encoding enterotoxins have been examined in order to study the potential pathogenicity of Bt to mammals and human beings. Methods Multiple polymerase chains reaction was performed to detect the hblA and bceT genes at the same time,the amplified fragments were 1071bp and 427bp,respectively,as we expected.The HBL blood agar was used to detect the hemolysin producing Bt strains. Results 29 Bt type strains(64.4%) and 10 Bt commercial strains(66.6%) have the hblA gene; 29 Bt type strains(64.4%) and 13 Bt commercial strains(86.6%) have the bceT gene. Identification of hemolysin producing Bt strains was conducted by the discontinuous hemolytic patterns on the HBL blood nutrient agar plates. 13 Bt type strains(28.8%) and 5 commercial strains(33.3%) produced the hemolysin BL. Conclusions The majority of Bt strains have hblA and bceT genes. In order to avoid the hazards to human beings, we suggest that the Bt strains which have no the two genes or the strains don't express the above genes can be used as the master strains for producing insecticides.

关 键 词：聚合酶链反应 苏芸金芽孢杆菌 肠毒素 基因 

分 类 号：R378.7[医药卫生—病原生物学]