顺序注射氢化物发生-原子荧光光谱法同时测定灵芝中As和Bi  

Simultaneous Determination on As and Bi in Ganoderma lucidum by Sequential Injection HydrideGeneration-Atom Fluorescence Spectrometry

作　　者：裴道国 徐文军[2] 

机构地区：[1]临沂出入境检验检疫局检验检疫技术中心,山东临沂276034 [2]临沂师范学院化学化工学院,山东临沂276005

出　　处：《安徽农业科学》2009年第35期17306-17308,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立1种顺序注射氢化物发生-原子荧光光谱法同时测定灵芝试样中的As和Bi含量的方法。[方法]灵芝样品采用微波快速消解系统消解,用绘制的标准曲线采用原子荧光光度计测得各个样品的As和Bi含量,并考察了光电倍增管负高压、As和Bi灯电流、原子化器高度、载气流量、屏蔽气流量等因素对测定结果的影响。[结果]载流HCl的浓度为0.3 mol/L,KBH4浓度为10 g/L时,同时测定As和Bi的效果最佳。在最佳试验条件下,As和Bi的检出限分别为0.079和0.031μg/L,加标回收率为90.5%～104.5%,相对标准偏差小于3.4%,灵芝试样中共存的离子对As和Bi的测定没有干扰。[结论]该方法操作方便、快速,灵敏性高,适用于灵芝试样品中As和Bi含量的同时测定,具有很好的可行性和实用性。[ Objective ] The study aimed to establish a method for simultaneous determination on As and Bi in Ganoderma lucidum samples by sequential injection hydride generation-atomic fluorescence spectrometry. [ Method ] G. lucidum samples were digested by microwave rapid di- gestion system and the contents of As and Bi in each sample were determined by atomic fluorescence spectrometer based on the drawn standard curve. The effects of the factors such as the negative high voltage on the photomuhiplier, lamp current of As and Bi, height of atomizer, carrier gas rate and shield gas rate on the determination of As and Bi were investigated. [ Result] When the concn, of hydrochloric acid and potassium borohydride were 0.3 mol/L and 10 g/L resp. , the effect of simultaneous determination on As and Bi was optimum. Under the optimum testing conditions, the detection limits of As and Bi were 0. 079 and 0. 031 μg/L resp. The standard addition recoveries for As and Bi were 90.5% - 104.5%, with the maximum RSD of 3.4%. The coexistence ions in determined G. lucidum samples didn＇ t interfere in the determination of As and Bi. [ Conclusion ] This determination method was convenient and rapid in operation with higher sensitivity, so it had good feasibility and nracticabilitv for simultaneous determination on As and Bi in G. lucidum samples.

关 键 词：顺序注射 氢化物发生 原子荧光光谱法 砷 铋 

分 类 号：S567.31[农业科学—中草药栽培]