印度橡胶榕离体培养及植株再生研究  

Study on in vitro Culture and Plant Regeneration of Ficus elastica

作　　者：陈泽雄[1] 刘奕清[1] 黄登艳[1] 

机构地区：[1]重庆文理学院花卉研究所重庆高校园林花卉工程研究中心,重庆402168

出　　处：《安徽农业科学》2009年第35期17350-17352,共3页Journal of Anhui Agricultural Sciences

基　　金：重庆市教委重大平台建设项目(GCZX0713)

摘　　要：[目的]为印度橡胶榕的工厂化育苗提供理论依据。[方法]以印度橡胶榕"黑金刚"茎尖为材料,以MS为基本培养基添加不同的激素组合进行组织培养,选择最佳的培养基配方。[结果]外植体初代诱导最佳培养基为:MS+6-BA2.0 mg/L+NAA0.1 mg/L,诱导率达69.7%;丛生芽增殖最佳培养基为:MS+6-BA1.0 mg/L+NAA0.1 mg/L,增殖系数达2.57;最佳生根培养基为:1/2 MS+NAA0.1mg/L+IBA0.1 mg/L,其生根率为100%。[结论]该研究成功建立了印度橡胶榕"黑金刚"的离体再生体系,为其规模化生产提供技术支撑。[ Objective ] Provided a theory foundation for industrialized breeding of Ficus elastica. [ Method ] Using shoot tip of Ficus elastica ＇ Decora Burgundy＇ as explants, Using MS as basic medium with different hormone combinations and selected the best medium combination to set up the plant regeneration System of Ficus elastica ＇ Decora Burgundy＇. [ Result] The optimum medium for primary induction of axillary buds was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.1 rag/L, the average induction ratio reach 69.7% , the optimum subculture medium for stem multiplication was MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.1 rag/L, on which each explants produced 2.57 plantlets on the average. The frequency of rooting reached 100% on 1/2 MS medium supplemented with NAA 0.1 mg/L and 0.1 mg/L IBA. [ Conclusion] In vitro culture and plant regeneration system of Ficus elastica ＇ Decora Burgundy＇ was established in this study and the establised system provided a technology foundation for large scale oroduction of plantlet.

关 键 词：印度橡胶榕 茎尖 离体培养 植株再生 

分 类 号：S722.37[农业科学—林木遗传育种]