广藿香组培繁殖技术的研究  

Study on Tissue Culture and Rapid Propagation of Patchouli

作　　者：刘玉安[1] 勾玉璠[2] 唐晓杰[1] 杨春波[1] 

机构地区：[1]北华大学林学院,吉林吉林132013 [2]吉林省大安市农业技术推广中心,吉林大安131300

出　　处：《安徽农业科学》2009年第35期17358-17359,共2页Journal of Anhui Agricultural Sciences

基　　金：北华大学科研项目(科合字2006-19)"长白山区5种药用植物资源评价与快速繁殖技术研究"

摘　　要：[目地]研究广藿香组培快繁技术。[方法]利用MS加入不同质量浓度6-苄基氨基腺嘌呤(6-BA)和吲哚丁酸(IBA),进行试验。[结果]结果表明,MS培养基加入0.5 mg/L6-BA和0.1 mg/L IBA时,产生的愈伤组织的量最多,且不定芽增殖效果最好。无根苗在1/2 MS+IBA1.5 mg/L上生根率最高;试管苗在珍珠岩+蛭石成活率最高。[结论]该试验可为广藿香的组培繁殖提供参考。[ Objective ] The technology of tissue culture and rapidly propagation of patchouli （Pogostemon cablin）was studied. [Method ] The experiment in the MS medium with different concentration of 6-benzyladenine （6-BA） and indolebtyric acid （IBA） for its culturing was conducted. [ Results] The results showed that there were more callus in the MS ＋ 6-BA 0.5 mg/L ＋ IBA 0. 1 mg/L and the number of adventitious buds was also more than that from other media. The good rooting medium was 1/2 MS ＋ IBA 1. 5 mg/L. The plantlets planted in the mixed substratum （pearlite and vermiculite, 1：1 ） had the highest survival rate. [ Conclusion] The reference for the patchouli plant culture was pro- vided through the experiment.

关 键 词：广藿香 组织培养 增殖 移栽 

分 类 号：S603.6[农业科学—园艺学]