球根海棠叶离体培养中培养基配方的筛选  

Study on the Selection of Medium for Begonia×tuberhybrida Voss Leaf Culture in vitro

作　　者：建德锋[1] 孙良岩 陈凯峰[1] 

机构地区：[1]吉林农业科技学院植物科学分院,吉林吉林132101 [2]辽宁省高速公路管理局,辽宁沈阳110003

出　　处：《安徽农业科学》2009年第35期17360-17361,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]筛选球根海棠叶培养中不同阶段的培养基配方。[方法]通过组织培养试验,从接种、继代增殖和生根培养3个阶段对球根海棠叶离体培养中的培养基配方进行筛选。[结果]在MS+6-BA1.00 mg/L+NAA0.20 mg/L+Ade 5.00 mg/L的培养基上接种诱导分化丛生芽,诱导成芽率达100%,在诱导分化培养基中添加6-BA有利于芽的形成,在配方中添加Ade能促使诱导;在MS+6-BA0.10mg/L+NAA0.01 mg/L的培养基上进行增殖,增殖系数高达40,且丛生芽整齐、健壮,最适用于生产;在1/2 MS+IAA0.80 mg/L培养基上生根壮苗,生根时间短、根系位置适宜、根系条数偏多,最为理想。[结论]筛选出了球根海棠叶离体培养时诱导阶段、增殖阶段和生根阶段的最佳培养基配方:在MS+6-BA1.00 mg/L+NAA0.20 mg/L+Ade 5.00 mg/L的培养基上接种诱导分化丛生芽,然后在MS+6-BA0.10 mg/L+NAA0.01 mg/L的培养基上快速增殖,在1/2 MS+IAA0.80 mg/L培养基上生根壮苗,再出瓶培养成生产用苗。[ Objective ] The selection of the optimum media for Begonia × tuberhybrida Voss leaf culture in vitro in different periods was ex- perimented. [ Method ] The induction, subculture and rooting medium for its culture were experimented, respectively. [ Results] In medium： MS ＋ 6-BA 1.00 mg/L ＋ NAA 0.20 mg/L ＋ Ade 5.00 rag/L, the induction rate of bud Begonia x tuberhybrida leaf culture was 100% ; 6- BA added in the medium was beneficial to bud formation and Ade added in the medium promoted the induction. The multiplication coefficient of bud was as high as 40% in the subculture medium ： MS ＋ 6-BA 0. 10 mg/L ＋ NAA 0.01 mg/L and the bud growth was order and strong, which was suitable for production practice. In rooting medium： 1/2 MS ＋ IAA 0.80 mg/L, the root regenerated was strong and multiple, and the time of rooting was short. [ Conclusion] The procedure of Begonia x tuberhybrida Voss leaf culture in vitro was as follows ： the bud was induced in the medium： MS ＋ 6-BA 1.00 mg/L ＋ NAA 0.20 mg/L ＋ Ade 5.00 mg/L; propagated in the medium： MS ＋ 6-BA 0.10 mg/L ＋ NAA 0. 01 mg/L and rooted in the medium ： 1/2 MS ＋ IAA 0.80 mg/L.

关 键 词：球根海棠 培养基 筛选 

分 类 号：S661.4[农业科学—果树学]