培养基组成对环草石斛试管苗生长的影响  

Effects of Medium Composition on the Growth of Dendrobium loddigesii Rolfe Tube-seedlings

作　　者：毛堂芬 刘作易[2,3] 朱国胜[1,2] 黄永会[1,2] 

机构地区：[1]贵州省生物技术研究所,贵州贵阳550006 [2]贵州省农业生物技术重点实验室,贵州贵阳550006 [3]贵州省农业科学院,贵州贵阳550006

出　　处：《安徽农业科学》2009年第35期17367-17368,共2页Journal of Anhui Agricultural Sciences

基　　金：贵州省中药现代化科技产业研究开发专项项目[黔科合中药专字(2003)45号]

摘　　要：[目的]降低生产环草石斛试管苗的成本,为大规模生产种苗提供理论技术依据。[方法]比较白砂糖和分析纯蔗糖、自来水和蒸馏水以及有机物、微量元素、NAA等各成分对环草石斛试管苗生长的影响。[结果]自来水和白砂糖代替蒸馏水和分析纯蔗糖对环草石斛试管苗的生长无显著影响,而更有利于苗的生长;去除培养基中的有机物和微量元素对试管苗生长无明显影响;铁盐和NAA是试管苗生长不可缺少的成分。[结论]在环草石斛的试管苗生产中,自来水可以代替蒸馏水,食用白砂糖可以代替分析纯蔗糖,同时也可以去除培养基中的有机物和微量元素等。[ Objective ] The research aimed to reduce the production cost of Dendrobium loddigesii Rolfe tube-seedlings and provide the theoretical technology basis for the large-scale production of seedlings. [ Method ] The effects of white granulated sugar, analytical sucrose, tap water, distilled water, organic matter, trace elements arid NAA on the growth of D. Ioddigesii tube-seedlings were compared. [ Result ] Tap water and white granulated sugar that substituted for distilled water and sucrose resp. had no significant effects on the growth of D. loddigesii tubeseedlings and were more favorable for the growth of seedlings. The removal of organic matter and trace elements had no obvious effect on the growth of seedlings. Malysite and NAA were indispensable components for the seedling growth. [ Conclusion] In the production of D. loddigesii tube-seedlings, tap water could substitute for distilled water and white granulated sugar could substitute for pure sucrose. And organic matter and elements and other substances could be removed from the culture medium.

关 键 词：环草石斛 试管苗 培养基成分 

分 类 号：S567.23[农业科学—中草药栽培]