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作 者:袁宜如[1] 李晓云[1] 邹峥嵘[2] 高光林[1] 王晓锋[1] 蔡飞[1]
机构地区:[1]九江学院,江西九江332005 [2]江西师范大学生命科学学院,江西南昌330022
出 处:《安徽农业科学》2009年第35期17434-17435,17459,共3页Journal of Anhui Agricultural Sciences
基 金:江西省教育厅科技项目资助(GJJ08436)
摘 要:[目的]研究不同浓度梯度的芒萁水提取物对玉米种子萌发和幼苗生长的影响。[方法]将经TTC检测具有较强活力的玉米种子分成空白组、不同浓度的芒萁水提液处理组置于恒温箱中培养3~5 d,统计不同处理方法下种子的萌发率,再分别取样,采用DNS试剂测定萌发玉米种子淀粉酶活性,采用蒽酮比色法测定萌发玉米种子可溶性糖含量。[结果]一定浓度芒萁水提液可降低萌发玉米种子淀粉酶活性及可溶性糖含量,且水提液溶度越高,抑制作用越明显;对于玉米幼苗生长则表现出高浓度抑制,低浓度促进的效应。[结论]该研究为芒萁化感作用的进一步研究提供了重要参考。[ Objective ] The research aimed to study the effects of water extracts from Dicranopteirs pedata on the seed germination and seed- ling growth of Zea mays. [ Method] The higher vigor seeds that had been selected by TI'C method were cultivated in constant temperature incubator. The amylase activities of seed germination was determined by DNS (3,5-dinitrosalicylic acid) reagent. The soluble sugar content was determined with the anthrone colorimetry method. [ Result] The results showed that a certain extent water extracts could reduce the amylase activities and the soluble sugar content. Low concentration extracts showed promoting function and high concentration extracts expressed inhibitory function on the seedling growth of Zea mays. [ Conclusion] This research can provide important reference for further study on the Dicranopteirs pedata allelopathy.
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