碳离子辐照对甜高粱幼苗保护酶同工酶表达的影响  

Effects of Carbon Ion Beam Irradiation on Expression of Protective Enzymes Isozymes in Sweet Sorghum Seedlings

作　　者：李岩[1,2] 董喜存[1] 李文建[1] 冯亮英[1,2] 刘青芳[1] 何金玉[1] 曲颖[1] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]兰州大学,甘肃兰州730000

出　　处：《安徽农业科学》2009年第35期17440-17441,17444,共3页Journal of Anhui Agricultural Sciences

基　　金：2008年度中国科学院近代物理研究所所长基金项目(0806230SZO);中国科学院科技支甘项目(0806300YDO);中国科学院西部之光人才培养计划项目(0906040XBO)

摘　　要：[目的]分析碳离子辐照对甜高粱幼苗叶片中保护酶同工酶的影响。[方法]以甜高粱品种KFJT-CK为对照,以经碳离子辐照选育的早熟突变株KFJT-1为试材,采用垂直板聚丙烯酰胺凝胶电泳法(PAGE)对幼苗叶片中的过氧化物酶(POD)、酯酶(EST)和超氧化物歧化酶(SOD)同工酶谱进行了分析。[结果]突变后代KFJT-1与对照KFJT-CK相比,POD同工酶酶谱基本相同,说明碳离子辐照并未使甜高粱POD同工酶的表达发生明显的变化;SOD同工酶酶谱的条带数相同,只是条带的颜色深浅有所差异,说明碳离子辐照仅使SOD同工酶的表达量有所差异;EST同工酶增加了1条新谱带,说明碳离子束辐照使甜高粱EST的表达发生了变异。[结论]碳离子辐照对甜高粱幼苗叶片中POD的影响很小,对SOD有较微弱的作用,而对EST的同工酶谱有很大的影响。[ Objcetive ] Analyze the effects of carbon ion beam irradiation on the expression of protective enzymes isozymes in sweet sorghum seedling leaves. [ Method ] Using KFJT-CK and early-maturity mutant KFJT-1 , cultivated from KFJT-CK that treated by carbon ion beam ,as experiment materials , the isozymes of peroxidase （POD） , esterase （EST） and superoxide dismutase （SOD） were analyzed through polyacrylamide gel electrophoresis（ PAGE ）. [ Result ] Comparing KFJT-1 with KFJT-CK, the POD isozyme electrophoretogram was much the same which indicted that the expression of POD was not changed apparently by carbon ion beam irradiation. As for SOD, . the bands number were the same, but the color of some bands varied, which showed that the expression of SOD isozyme was slightly affected . The electrophoretogram of EST isozymes generated a new band , suggesting that the expression of EST in sweet sorghum was affected largely by carbon ion beam. [ Conclusion] carbon ion beam irradiation had no much effect on the expression POD isozymes, slight effect on the SOD expression and significant variation on the expression of EST isozymes.

关 键 词：甜高粱 碳离子辐照 同工酶 过氧化物酶 酶酯 超氧化物歧化酶 

分 类 号：S514[农业科学—作物学]