中性红染色法检测人参皂苷及其衍生物对CEF增殖的影响  

Effects of Ginsenoside and Its Derivatives on Proliferation of CEF Determined by Neutral Red Dye Absorption Method

作　　者：苏建青[1] 褚秀玲[1] 李俊霞[1] 付本懂[2] 申海青[2] 韦旭斌[2] 

机构地区：[1]聊城大学农学院,山东聊城252000 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《安徽农业科学》2009年第35期17523-17525,17527,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30471272)

摘　　要：[目的]为体外研制人参皂苷及其衍生物的抗MDV作用机制提供理论依据。[方法]采用中性红染料吸收法,检测了人参皂苷及其衍生物对鸡胚成纤维细胞(CEF)体外培养中增殖活性的影响。[结果]各药物的促增殖作用不完全相同,低毒性的药物促增殖作用更明显。从药物对CEF细胞的不同作用时间点来看,以72 h的OD值和正常对照的差异最大,在24 h和正常对照组差异不显著。[结论]人参皂苷及其衍生物在中、低浓度能够促进CEF细胞的增殖,且药物作用具有时间依赖性。[ Objective ] The research aimed to provide theoretical basis for studying anti-MDV mechanism of ginsenoside and its derivatives in vitro. [ Method] Effects of ginsenoside and its derivatives on proliferation activity of chick embryo throblast （CEF） in vitro were determined by using neutral red dye absorption method. [ Result ] The results showed the proliferation effects of different drugs are not completely same, and are more obvious with low toxic drugs. Seeing from different action time, the differences of OD value was biggest at 72 h when compared with normal control group, while there was no significant difference at 24 h. [ Conclusion] Ginsenoside and its derivatives could promote the proliferation of CEF cells in medium and low concentrations, which have time-dependent characteristic.

关 键 词：人参皂苷 衍生物 鸡胚成纤维细胞 安全浓度 

分 类 号：S859.3[农业科学—临床兽医学]