接种根瘤菌对豌豆根际细菌数量动态变化及产量的影响  

Effect of inoculated peas with rhizobium on the number of pea rhizobacteria dynamic changes and yields

作　　者：马剑[1] 黄高宝[2] 高亚琴[1] 王晓娟[1] 

机构地区：[1]甘肃农业大学资源与环境学院,甘肃兰州730070 [2]甘肃农业大学农业生态工程研究所,甘肃兰州730070

出　　处：《干旱地区农业研究》2009年第6期7-10,共4页Agricultural Research in the Arid Areas

基　　金：国家科技支撑计划项目(2007BAD89B17);甘肃省自然基金(3ZS061-A25-079)

摘　　要：采用根箱法对豌豆接种根瘤菌,用尼龙筛网将根系及离根不同水平距离的土壤分层隔开,研究豌豆根际细菌的动态变化以及与产量的关系。结果表明:(1)接种匹配性好的根瘤菌剂可以提高土壤中根际细菌数量与豌豆产量,接种ACCC16103根瘤菌豌豆单株产量相对不接种提高30.6%,根际细菌数量增加了117.5%,而接种ACCC16101,豌豆单株产量下降6.8%,根际细菌数量在盛花期和结荚期低于不接种,仅成熟期高于不接种。(2)根际细菌数量表现明显的根面聚集现象。(3)盛花期豌豆根际细菌数量是决定豌豆高产的主要因素之一,豌豆的产量与结荚期根际细菌数量呈显著正相关关系(r=0.768),与盛花期根际细菌数量呈极显著正相关关系(r=0.865),与成熟期根际细菌数量呈正相关关系(r=0.414),但未达到显著水平。Rhizosphere bacteria dynamic changes and the relations between rhizosphere bacteria and yields were studied by root box method which divided stratifiedly the root system and soil at different distances from the root.The results show that： （1） Inoculated peas with better matching rhizobium can increase the rhizosphere bacteria of soil and yield of peas.Compared with CK treatment,the yield of peas in inoculated ACCC16103 increases by 30.6% and the number of pea rhizosphere bacteria increases by 117.5%.The number of rhizosphere bacteria is less than CK in flowering stage and seed pumping stage,but more than CK in mature stage and the yield of pea declines by 6.8%.（2） The rhizosphere bacteria shows obviously accumulated in root surface.（3） The number of pea rhizosphere bacteria in flowering stage is one of the main factors to determine the pea yield,and there is significantly positive correlation（r=0.768） between the number of pea rhizobacteria in flowering stage and pea yield,and there is positively correlation（r=0.4140） between the number of pea rhizobacteria in mature stage and pea yield,but does not reach significant level.

关 键 词：豌豆 根瘤菌 根际细菌 

分 类 号：S154.381[农业科学—土壤学]