利用SRAP-BSA法筛选棉花抗病基因的分子标记  

Molecular Markers of Screening of the Disease-resistance Gene of Cotton Using SRAP-BSA Technique

作　　者：刘艳[1,2,3] 陈全家[1,2,3] 曲延英[1,2,3] 梁亚军[1,2,3] 李培玉[1,2,3] 朱彪[1] 吕玉强[1] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业大学农业技术重点实验室,乌鲁木齐830052 [3]教育部棉花工程中心．乌鲁木齐830052

出　　处：《新疆农业科学》2009年第6期1158-1163,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高新技术项目(20080101);新疆维吾尔自治区教育厅重点项目(XJEDU2007I15)

摘　　要：【目的】以高抗黄萎病品种辽棉18号和高感黄萎病品种军棉1号及其杂交后代F2为材料,筛选棉花抗病基因。【方法】采用分离群体分组分析(Bulked Segregate Analysis,BSA)法,对棉花抗病基因进行SRAP分析。【结果】在88对SRAP引物中筛选出3对引物在两亲本及抗、感基因池中均能扩增出稳定的差异条带,命名为S6-6、S6-10、S8-8。【结论】通过F2代个体验证,抗病个体均能扩增出此差异条带,而感病个体中不能扩增出此差异条带,证明这三对引物是与棉花抗病基因紧密连锁的SRAP分子标记。【Objective and Method】In this experiment,the upland cotton resistant parent Liaomian 18 and sensitive parent Junmian 1 and their F2 generation were used for identification of the molecular markers linked to disease-resistance gene of cotton by SRAP-BSA,【Result】88 pairs of primers were used to amplify the parents gene and two gene pools.When 3 pairs of primers were found in parents and two pools,three pairs of primers were screened from 88 paris of SRAP primers.【Conclusion】The fragment existed in the resistant plants and F population,they were indicated that the SRAP marker were closely linked to disease-resistance gene of cotton.

关 键 词：棉花 抗病基因 SRAP BSA 

分 类 号：S562.035.3[农业科学—作物学]