新疆小芦苇青贮中乳酸杆菌的分离鉴定  

Isolation and Identification of Lactobacillus from Xinjiang Small Reed Silage

作　　者：张想峰[1] 艾尼瓦尔.艾山[1] 买热木尼沙.吾甫尔 闫京阳 于苏甫.热西提 

机构地区：[1]新疆农业大学动物营养实验室,乌鲁木齐830052 [2]巴音郭楞蒙古自治州动物疾病控制与诊断中心,新疆库尔勒841000 [3]塔城地区畜牧科技研究推广中心,新疆塔城834700

出　　处：《新疆农业科学》2009年第6期1327-1331,共5页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重大专项项目"奶牛产业技术开发与集成示范"(200731134);国家科技支撑计划项目"西北农区生态型奶业生产技术和奶牛性控技术研究与开发"(2006BAD04A11-06);农业部奶产业技术体系项目

摘　　要：【目的】筛选新疆小芦苇青贮中优良的乳酸杆菌。【方法】研究将从自制的新疆小芦苇青贮中分离出乳酸杆菌进行鉴定,对鉴定出的菌株进行产酸试验,确定新疆小芦苇青贮中的优势菌株。【结果】试验共分离得到了11株乳酸杆菌,根据《伯杰氏细菌鉴定手册》(第八版)中的种属表述结果,经鉴定2株(Hz2-1和Hz2-2)为戊糖乳杆菌(L.pentosus),2株(Hz3-1和Hz3-2)为植物乳杆菌(L.plantarum),2株(Zar2-8和Zar2-9)为牛粪乳杆菌(L.dung),2株(Zar8-3和Zar8-4)为短乳杆菌(L.brevis),3株(Gsr1-1、Hz1-1和Zar9-4)为德氏乳杆菌保加利亚亚种(L.delbrueckiisubsp.bulgaricus)。【结论】从不同菌株发酵产酸试验的结果来看,发现Hz2-1,Hz3-1具有较强的产酸能力,pH最低可达3.50,是新疆小芦苇青贮发酵过程中的主要产酸菌株。【Objective】Xinjiang small reed distributed widely and which is good forage resources of ruminants.【Method】This study will isolate and identify Lactobacillus from the self-regulating Xinjiang small reed silage.The trains were acidogenic tests that identified the advantages trains of Xinjiang small reed silage.11 strains of Lactobacillus were isolated from the self-regulating reed silage of different origin.According to the criteria and description of Bergey＇s manual of systematic bacteriology（The eighth edition）,which include two strains Lactobacillus pentosus（Hz2-1,Hz2-2）,two strains Lactobacillus plantarum（Hz3-1,Hz3-2）,two strains lactobacillus dung（Zar2-8,Zar2-9）,two strains Lactobacillus brevis（Zar8-3,Zar8-4）,three strains Lactobacillus delbrueckii subsp.bulgaricus（Gsr11,Hz1-1,Zar9-4） were identified.Based on a strain for the fermentation acid test,it was found that a strong acid production capacity were observed from HZ2-1,HZ3-1,the pH up the minimum could reach 3.50,which was the main strains from fermentation process of Xinjiang small reed.

关 键 词：新疆小芦苇 青贮 乳酸杆菌 分离鉴定 

分 类 号：S188[农业科学—农业基础科学]