3种方法建立小鼠脾虚模型过程中血清褪黑激素水平变化规律的研究  

Change of serum melatonin levels in the course of modeling three types of Spleen difficiency model in mice

作　　者：凌智群[1] 周艳霞 王泽芬[3] 汤小虎[4] 吴兵[1] 江道峰[1] 范成杰[1] 万丽[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]云南新兴职业学院,云南昆明650501 [3]武汉大学基础医学院生理系,湖北武汉430072 [4]云南省中医医院风湿科,云南昆明650021

出　　处：《中国中西医结合消化杂志》2009年第6期358-360,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：四川省青年科技基金(07ZQ026-031)

摘　　要：[目的]研究3种方法建立小鼠脾虚模型过程中血清褪黑激素(MT)水平的变化规律,进一步探讨MT在脾虚证中的作用。[方法]利用ELISA法检测血清MT水平,观察MT在建立利血平型、大黄型和偏食醋型小鼠脾虚模型过程中的变化规律。[结果]随造模时间的延长,3种脾虚模型动物血清MT水平变化过程明显不同,但文献中显示的3种脾虚模型建立成功天数,与笔者实验中MT水平低下所对应的天数恰好吻合。[结论]提示MT的改变较普遍地存在于脾虚的发病过程中,不仅可以作为某些脾虚证的特征参照指标,也可能是某些脾虚病症在某个阶段的的重要客观生化指标。[Objective]To investigate the relation of splenasthenic syndrome and serum melatonin （MT） levels in spleen deficiency model mice with different mechanisms. [Methods]Serum MT levels in three types of spleen deficiency mice were detected at 01：00-02： 00AM by means of ELISA method, and the change trends were observed during the course of modeling spleen deficiency mice, including Reserpine ,Vinegar eating only and Rhubarb type. [Results]During the modeling course, the serum MT levels in both reserpine-treated and vinegar-treated dropped down gradually, but that in Rhubarb-treated mice at first decreased significantly and regain the normal level before lowering markedly. [Conclusion]As a biological parameter,MT can play an important role in spleen deficiency,or in the certain stage Spleen dificiency disease.

关 键 词：脾虚 褪黑激素 

分 类 号：R256.39[医药卫生—中医内科学]